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First published online August 3, 2006
Journal of Experimental Biology 209, 3241-3256 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02372

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Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs of Cancer crabs: implications for differential release and pleiotropic function

Yun-Wei A. Hsu¹, Daniel I. Messinger¹, J. Sook Chung^2,3, Simon G. Webster², Horacio O. de la Iglesia¹ and Andrew E. Christie^1,4,*

¹ Department of Biology, University of Washington, Box 351800, Seattle, WA 98195-1800, USA
² School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, UK
³ Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
⁴ Friday Harbor Laboratories, University of Washington, 620 University Road, Friday Harbor, WA 98250, USA

^* Author for correspondence (e-mail: crabman{at}u.washington.edu)

Accepted 6 June 2006

The crustacean hyperglycemic hormone (CHH) family of peptides includes CHH, moult-inhibiting hormone (MIH) and mandibular organ-inhibiting hormone (MOIH). In the crab Cancer pagurus, isoforms of these peptides, as well as CHH precursor-related peptide (CPRP), have been identified in the X-organ-sinus gland (XO-SG) system. Using peptides isolated from the C. pagurus SG, antibodies to each family member and CPRP were generated. These sera were then used to map the distributions and co-localization patterns of these peptides in the neuroendocrine organs of seven Cancer species: Cancer antennarius, Cancer anthonyi, Cancer borealis, Cancer gracilis, Cancer irroratus, Cancer magister and Cancer productus. In addition to the XO-SG, the pericardial organ (PO) and two other neuroendocrine sites contained within the stomatogastric nervous system, the anterior cardiac plexus (ACP) and the anterior commissural organ (ACO), were studied. In all species, the peptides were found to be differentially distributed between the neuroendocrine sites in conserved patterns: i.e. CHH, CPRP, MIH and MOIH in the XO-SG, CHH, CPRP and MOIH in the PO, and MOIH in the ACP (no immunolabeling was found in the ACO). Moreover, in C. productus (and probably in all species), the peptides present in the XO-SG and PO were differentially distributed between the neurons within each of these neuroendocrine organs (e.g. CHH and CPRP in one set of XO somata with MIH and MOIH co-localized in a different set of cell bodies). Taken collectively, the differential distributions of CHH family members and CPRP both between and within the neuroendocrine organs of crabs of the genus Cancer suggests that each of these peptides may be released into the circulatory system in response to varied, tissue-specific cues and that the PO- and/or ACP-derived isoforms may possess functions distinct from those classically ascribed to their release from the SG.

Key words: Cancer crabs, sinus gland, (SG), pericardial organ (PO), anterior cardiac plexus (ACP), anterior commissural organ (ACO), stomatogastric nervous system (STNS), stomatogastric ganglion (STG), neurohormone, crustacean hyperglycemic hormone (CHH), crustacean hyperglycemic hormone precursor-related peptide (CPRP), mandibular organ-inhibiting hormone (MOIH), moult-inhibiting hormone (MIH), immunohistochemistry