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First published online March 21, 2005
Journal of Experimental Biology 208, 1393-1399 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01512

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Undetectable apolipoprotein A-I gene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine of Cyprinus carpio

Margarita I. Concha, Rodrigo López, Julieta Villanueva, Nadya Báez and Rodolfo Amthauer^*

Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile

^* Author for correspondence (e-mail: ramthaue{at}uach.cl)

Accepted 26 January 2005

High density lipoprotein (HDL) has been shown to play an important role in the dietary lipid mobilisation in the carp. In spite of this, previous studies have failed to demonstrate the synthesis of the major protein component of HDL, apolipoprotein A-I (apoA-I), in the proximal intestine of the carp. Therefore, the aim of the present study was to evaluate the expression of apoA-I throughout the entire intestine. Curiously, no transcription of the apoA-I gene could be detected either by northern blot or RT–PCR assays in the intestinal mucosa, in clear contrast with the abundant cytosolic immunoreactive apoA-I detected in almost all intestinal segments, which suggests a different origin for this protein. In addition, the detection of specific, but low affinity, binding sites for apoA-I in the carp intestinal brush-border membranes (BBM), and the strong interaction with BBM, which is highly dependent on temperature, points to an important contribution of membrane lipids in apoA-I binding to the intestinal mucosa. This idea was reinforced by the ability of carp apoA-I to associate with multilamellar phospholipid vesicles.

Key words: apoA-I, internalisation, lipid absorption, carp, Cyprinus carpio

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