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First published online January 5, 2005
Journal of Experimental Biology 208, 345-354 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01382
Regulation of branchial V-H+-ATPase, Na+/K+-ATPase and NHE2 in response to acid and base infusions in the Pacific spiny dogfish (Squalus acanthias)
Dept of Biological Sciences, University of Alberta, Edmonton, Alberta T5G 2E9, Canada and Bamfield Marine Research Centre, Bamfield, BC V0R 1B0, Canada
* Author for correspondence (e-mail: martint{at}ualberta.ca)
Accepted 11 November 2004
To study the mechanisms of branchial acid-base regulation, Pacific spiny
dogfish were infused intravenously for 24 h with either HCl (495± 79
µmol kg-1 h-1) or NaHCO3 (981±235
µmol kg-1 h-1). Infusion of HCl produced a transient
reduction in blood pH. Despite continued infusion of acid, pH returned to
normal by 12 h. Infusion of NaHCO3 resulted in a new steady-state
acid-base status at 
0.3 pH units higher than the controls. Immunostained
serial sections of gill revealed the presence of separate vacuolar proton
ATPase (V-H+-ATPase)-rich or sodium-potassium ATPase
(Na+/K+-ATPase)-rich cells in all fish examined. A
minority of the cells also labeled positive for both transporters. Gill cell
membranes prepared from NaHCO3-infused fish showed significant
increases in both V-H+-ATPase abundance (300±81%) and
activity. In addition, we found that V-H+-ATPase subcellular
localization was mainly cytoplasmic in control and HCl-infused fish, while
NaHCO3-infused fish demonstrated a distinctly basolateral staining
pattern. Western analysis in gill membranes from HCl-infused fish also
revealed increased abundance of Na+/H+ exchanger 2
(213±5%) and Na+/K+-ATPase (315±88%)
compared to the control.
Key words: dogfish, Squalus acanthias, gill, acid-base regulation, H+-ATPase, Na+/K+-ATPase, NHE, acid infusion, base infusion, alkalosis, acidosis
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