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First published online September 16, 2005
Journal of Experimental Biology 208, 3627-3636 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01820
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Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus

Carlos M. Luquet1,2,*, Dirk Weihrauch3, Mihaela Senek4 and David W. Towle5,{dagger}

1 Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
2 CONICET (Consejo Nacional de Investigaciones Cientificas y Tecnicas), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
3 Department of Animal Physiology, University of Osnabrueck, 49076 Osnabrueck, Germany
4 College of the Atlantic, Bar Harbor, ME 04609, USA
5 Mount Desert Island Biological Laboratory, Salsbury Cove, ME 04672, USA

{dagger} Author for correspondence (e-mail: dtowle{at}mdibl.org)

Accepted 1 August 2005

Using quantitative real-time PCR, the expression of mRNAs encoding three transport-related proteins and one putative housekeeping protein was analyzed in anterior and posterior gills of the euryhaline crab Chasmagnathus granulatus following transfer from isosmotic conditions (30{per thousand} salinity) to either dilute (2{per thousand}) or concentrated (45{per thousand}) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H+-ATPase B-subunit, both of which were highly expressed under all conditions. By contrast, the expression of Na+/K+-ATPase {alpha}-subunit mRNA and Na+/K+/2Cl- cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10-20-fold in posterior gills within the first 24 h while Na+/K+-ATPase {alpha}-subunit mRNA increased 35-55-fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96 h and Na+/K+-ATPase {alpha}-subunit increased approximately 25-fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.

Key words: Na+/K+-ATPase, Na+/K+/2Cl- cotransporter, V-type H+-ATPase, arginine kinase, crab, gill, gene expression, quantitative PCR




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