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First published online September 16, 2005
Journal of Experimental Biology 208, 3627-3636 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01820

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Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus

Carlos M. Luquet^1,2,*, Dirk Weihrauch³, Mihaela Senek⁴ and David W. Towle^5,

¹ Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
² CONICET (Consejo Nacional de Investigaciones Cientificas y Tecnicas), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
³ Department of Animal Physiology, University of Osnabrueck, 49076 Osnabrueck, Germany
⁴ College of the Atlantic, Bar Harbor, ME 04609, USA
⁵ Mount Desert Island Biological Laboratory, Salsbury Cove, ME 04672, USA

Author for correspondence (e-mail: dtowle{at}mdibl.org)

Accepted 1 August 2005

Using quantitative real-time PCR, the expression of mRNAs encoding three transport-related proteins and one putative housekeeping protein was analyzed in anterior and posterior gills of the euryhaline crab Chasmagnathus granulatus following transfer from isosmotic conditions (30 salinity) to either dilute (2) or concentrated (45) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H⁺-ATPase B-subunit, both of which were highly expressed under all conditions. By contrast, the expression of Na⁺/K⁺-ATPase -subunit mRNA and Na⁺/K⁺/2Cl^- cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10-20-fold in posterior gills within the first 24 h while Na⁺/K⁺-ATPase -subunit mRNA increased 35-55-fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96 h and Na⁺/K⁺-ATPase -subunit increased approximately 25-fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.

Key words: Na⁺/K⁺-ATPase, Na⁺/K⁺/2Cl^- cotransporter, V-type H⁺-ATPase, arginine kinase, crab, gill, gene expression, quantitative PCR

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