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First published online August 4, 2005
Journal of Experimental Biology 208, 3211-3218 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01756
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A chemotactic response facilitates mosquito salivary gland infection by malaria sporozoites

Mayumi Akaki and James A. Dvorak*

Biochemical and Biophysical Parasitology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Bethesda, MD 20892-8132, USA

* Author for correspondence (e-mail: jdvorak{at}niaid.nih.gov)

Accepted 15 June 2006

Sporozoite invasion of mosquito salivary glands is critical for malaria transmission to vertebrate hosts. After release into the mosquito hemocoel, the means by which malaria sporozoites locate the salivary glands is unknown. We developed a Matrigel-based in vitro system to observe and analyze the motility of GFP-expressing Plasmodium berghei sporozoites in the presence of salivary gland products of Anopheles stephensi mosquitoes using temperature-controlled, low-light-level video microscopy. Sporozoites moved toward unheated salivary gland homogenate (SGH) but not to SGH that had been heated at 56°C for 30 min. We also investigated the origin of the attracted population. Attraction to SGH was restricted to hemolymph- and oocyst-derived sporozoites; salivary gland-derived sporozoites were not attracted to SGH. These data imply that sporozoites employ a chemotactic response to high molecular mass proteins or carbohydrate-binding proteins to locate salivary glands. This raises the possibility of utilizing anti-chemotactic factors for the development of mosquito transmission blocking agents.

Key words: malaria, sporozoite, salivary gland, chemotaxis, mosquito


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MOSQUITO SALIVARY GLANDS LURE MALARIA PARASITES
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JEB 2005 208: i. [Full Text]  



This article has been cited by other articles:


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Y. van Bergen
MOSQUITO SALIVARY GLANDS LURE MALARIA PARASITES
J. Exp. Biol., August 15, 2005; 208(16): i - i.
[Full Text] [PDF]


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