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First published online June 6, 2005
Journal of Experimental Biology 208, 2409-2420 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01632

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Temperature-dependent protein synthesis capacities in Antarctic and temperate (North Sea) fish (Zoarcidae)

Daniela Storch^*, Gisela Lannig and Hans O. Pörtner

Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, D-27570 Bremerhaven, Germany

^* Author for correspondence at present address: Animal Ecophysiology, Alfred-Wegener-Institut für Polar- und Meeresforschung, Am Handelshafen 12, D-27570 Bremerhaven (e-mail: dstorch{at}awi-bremerhaven.de)

Accepted 5 April 2005

For an evaluation of effects of seasonal cold acclimation and evolutionary cold adaptation on protein synthesis capacity, the protein synthesis apparatus was isolated from the gills and white muscle of Antarctic eelpout Pachycara brachycephalum and North Sea eelpout Zoarces viviparus. Both species had been acclimated to 0°C (control) and 5°C (Antarctic) and 5°C and 10°C (North Sea control). The translational capacities of the protein synthesis machineries were determined in an optimised cell-free in vitro system. The results demonstrate that tissues from the polar zoarcid possess cold-adapted protein synthesis machineries, indicated by low activation energies and, especially, high RNA translational capacities at similar RNA:protein ratios when compared to temperate zoarcids at 10°C. When both species were brought to 5°C, the temperate species displayed cold compensated protein synthesis capacities caused by elevated RNA:protein ratios. Warm exposure (from 0 to 5°C) of the Antarctic zoarcid revealed a capacity for thermal acclimation indicated by a reduction in protein synthesis capacities associated with lower RNA:protein ratios.

Key words: RNA translational capacity, RNA:protein ratio, Zoarces viviparus, Pachycara brachycephalum, cold acclimation, cold adaptation

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