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First published online May 5, 2005
Journal of Experimental Biology 208, 1915-1925 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01570

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Genomic sequences encoding two types of medaka hemopexin-like protein Wap65, and their gene expression profiles in embryos

Makiko Nakaniwa¹, Makoto Hirayama¹, Atsushi Shimizu², Takashi Sasaki², Shuichi Asakawa², Nobuyoshi Shimizu² and Shugo Watabe^1,*

¹ Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo113-8657, Japan
² Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan

^* Author for correspondence (e-mail: awatabe{at}mail.ecc.u-tokyo.ac.jp)

Accepted 7 March 1005

Medaka genomic BAC clones, which contained two types of medaka hemopexin-like protein gene (Wap65), mWap65-1 and mWap65-2, were screened and their genomic sequences were determined by the shotgun strategy. The exon-intron organizations were highly conserved between both mWap65s and human hemopexin genes. The 5'-flanking regions of mWap65-1 and mWap65-2 contained various putative transcription factor binding sites including elements for developmental regulation. The expression patterns of mWap65s during embryonic development were examined by quantitative real-time PCR, demonstrating that both mWap65 transcripts were observed in early embryonic stages, but their expression patterns were different. Interestingly, in situ hybridization revealed that mWap65-2 transcripts were restricted to liver, whereas mWap65-1 transcripts were detected along the edge of pectoral fin buds and the median fin fold of tail buds in embryos at stage 32. Furthermore, we generated transgenic medaka expressing GFP driven by mWap65-1 and mWap65-2 promoters and observed GFP expression patterns during ontogeny. Although localizations of GFP varied among individuals, embryos uniformly expressed GFP 1 day after injection of mWap65-1-hrGFP and mWap65-2-hrGFP constructs, suggesting that mWap65-1 and mWap65-2 promoters were activated in very early stages. The differences between mWap65-1 and mWap65-2 in their expression profiles indicate their distinct roles during ontogeny.

Key words: medaka, Oryzias latipes, mWap65-1, mWap65-2, hemopexin, genomic sequences, embryo, gene expression, transgenic fish

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