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First published online March 22, 2004
Journal of Experimental Biology 207, 1533-1542 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00919
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ß-naphthoflavone induction of CYP1A in brain of juvenile lake trout (Salvelinus namaycush Walbaum)

Yu-Wen Chung-Davidson, Christopher B. Rees, Hong Wu, Sang-Seon Yun and Weiming Li*

Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA

* Author for correspondence (e-mail: liweim{at}msu.edu)

Accepted 3 February 2004

Many environmental pollutants induce expression of the cytochrome P450 (CYP) 1A subfamily of genes. We integrated cellular and molecular biological techniques to examine the effects of ß-naphthoflavone (BNF) exposure in lake trout brain CYP1A distribution and dynamics. Over a 32-day time-course, real time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) results showed that CYP1A mRNA induction in response to BNF exposure occurred rapidly and continued to rise in the BNF-treated lake trout after 4 h, with a peak at or after 2 days. Messenger RNA levels fell after 4 days, and this trend continued after 16 days of exposure. In situ hybridization indicated that CYP1A mRNA was universally elevated in the brain of BNF-exposed fish and was mainly expressed in the endothelia and occasionally in the glial cells. CYP1A immunoreactivity was induced in the olfactory bulb and valvula cerebelli of BNF-treated fish. Other brain areas showed constitutive CYP1A immunoreactivity in both control and BNF-treated fish. Some BNF-treated fish contained multifocal hemorrhages in the brain tissue, and these fish had overall depressed CYP1A immunoreactivity in the brain. The relationship between transcriptional and translational effects of BNF exposure in the brain of juvenile lake trout is discussed.

Key words: lake trout, Salvelinus namaycush, CYP1A induction, brain, cytochrome P450, ß-naphthoflavone


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© The Company of Biologists Ltd 2004