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First published online February 20, 2004
Journal of Experimental Biology 207, 1101-1111 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00858
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Stimulation by cadmium of myohemerythrin-like cells in the gut of the annelid Nereis diversicolor

Sylvain Demuynck1,*, Beatrice Bocquet-Muchembled2, Laurence Deloffre2, Fabien Grumiaux1 and Alain Leprêtre1

1 Laboratoire d'Ecologie Numérique et d'Ecotoxicologie UPRES EA 3570, FR 1818 CNRS
2 Laboratoire de Neuroimmunité des Annélides UMR 97, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq Cedex, France

* Author for correspondence (e-mail: sylvain.demuynck{at}univ-lille1.fr)

Accepted 5 January 2004

Isolated guts of Nereis diversicolor revealed the existence of a cadmium-binding protein, the MPII, belonging to the group of hemerythrins and myohemerythrins. The presence of MPII in the cells of the intestine was demonstrated by immunocytochemistry, using anti-MPII, a monoclonal antibody. In addition, using in situ hybridization and northern blotting, it was shown that MPII-cells are the site of synthesis of this molecule. Exposure of the worms to cadmium led to the cellular activation process of MPII-cells (i.e. transformation of the nucleolus, development of the endoplasmic reticulum and the Golgi apparatus), although MPII mRNA transcript levels were unchanged. Enzyme-linked immunosorbent assay (ELISA) of gut extracts revealed that MPII levels were increased after exposure to Cd, so it appears that this protein is synthesized as a response to Cd exposure without any new synthesis of mRNA. This mechanism of regulation is quite similar to that reported in the case of mammalian ferritin and may be involved in the regulation of Cd levels in this worm.

Key words: annelid, Nereis diversicolor, myohemerythrin, MPII, cadmium-binding protein, midgut


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