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First published online February 6, 2004
Journal of Experimental Biology 207, 905-912 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00844
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Seawater acclimation causes independent alterations in Na+/K+- and H+-ATPase activity in isolated mitochondria-rich cell subtypes of the rainbow trout gill

Guy S. Hawkings, Fernando Galvez* and Greg G. Goss{dagger}

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6E 4W1

{dagger} Author for correspondence (e-mail: greg.goss{at}ualberta.ca)

Accepted 17 December 2003

Mitochondria-rich cells (MR cells) of the gills of rainbow trout undergo changes in relative distribution and biochemical function during acclimation to partial-strength (10{per thousand}) and full-strength (30{per thousand}) seawater. In isolated total gill cells, Na+/K+-ATPase activity increased fivefold and H+-ATPase activity decreased fourfold when trout were acclimated to either 10{per thousand} or 30{per thousand} seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative distribution in seawater-acclimated fish. In freshwater, the ratio of PNA–:PNA+ was 65:35 while in seawater the distribution changed to 20:80 PNA–:PNA+. Additionally, differential changes in Na+/K+-ATPase and H+-ATPase activity in each of the independent cell types occurred during seawater acclimation; Na+/K+-ATPase activity in the PNA– cells increased by 197% while in PNA+ cells Na+/K+-ATPase decreased by 57%. However, H+-ATPase activity was decreased in both PNA– (84%) and PNA+ (72%) subtypes during acclimation to seawater.

Key words: mitochondria-rich cells, MR cells, sodium, transport, Na+/K+-ATPase, H+-ATPase, rainbow trout, Oncorhynchus mykiss, gill, seawater adaptation


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© The Company of Biologists Ltd 2004