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First published online February 6, 2004
Journal of Experimental Biology 207, 905-912 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00844

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Seawater acclimation causes independent alterations in Na⁺/K⁺- and H⁺-ATPase activity in isolated mitochondria-rich cell subtypes of the rainbow trout gill

Guy S. Hawkings, Fernando Galvez^* and Greg G. Goss

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6E 4W1

Author for correspondence (e-mail: greg.goss{at}ualberta.ca)

Accepted 17 December 2003

Mitochondria-rich cells (MR cells) of the gills of rainbow trout undergo changes in relative distribution and biochemical function during acclimation to partial-strength (10) and full-strength (30) seawater. In isolated total gill cells, Na⁺/K⁺-ATPase activity increased fivefold and H⁺-ATPase activity decreased fourfold when trout were acclimated to either 10 or 30 seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative distribution in seawater-acclimated fish. In freshwater, the ratio of PNA^–:PNA⁺ was 65:35 while in seawater the distribution changed to 20:80 PNA^–:PNA⁺. Additionally, differential changes in Na⁺/K⁺-ATPase and H⁺-ATPase activity in each of the independent cell types occurred during seawater acclimation; Na⁺/K⁺-ATPase activity in the PNA^– cells increased by 197% while in PNA⁺ cells Na⁺/K⁺-ATPase decreased by 57%. However, H⁺-ATPase activity was decreased in both PNA^– (84%) and PNA⁺ (72%) subtypes during acclimation to seawater.

Key words: mitochondria-rich cells, MR cells, sodium, transport, Na⁺/K⁺-ATPase, H⁺-ATPase, rainbow trout, Oncorhynchus mykiss, gill, seawater adaptation

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