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First published online December 22, 2003
Journal of Experimental Biology 207, 519-526 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00772

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Effects of leucokinin-VIII on Aedes Malpighian tubule segments lacking stellate cells

Ming-Jiun Yu and Klaus W. Beyenbach^*

Department of Biomedical Sciences, VRT 8004, Cornell University, Ithaca, NY 14853, USA

^* Author for correspondence (e-mail: kwb1{at}cornell.edu)

Accepted 27 October 2003

The diuretic peptide leucokinin is known to increase fluid secretion in Malpighian tubules of the yellow fever mosquito Aedes aegypti by increasing a transepithelial Cl^- conductance. The present study sought to examine whether stellate cells provided this transepithelial conductance in Aedes Malpighian tubules as they do in Drosophila Malpighian tubules. Aedes Malpighian tubule segments with and without stellate cells were perfused in vitro for measurements of the transepithelial voltage (V_t), resistance (R_t) and Cl^- diffusion potentials (DP_Cl). In 11 tubule segments containing both principal cells and stellate cells, 1 µmol l^-1 leucokinin-VIII added to the peritubular bath immediately and significantly decreased V_t from 39.3±14.3 mV to 2.3±0.7 mV, decreased R_t from 12.4±2.6 kcm to 2.4±0.3 kcm, and increased DP_Cl from 8.2±1.2 mV to 42.1±5.4 mV. These effects of leucokinin-VIII were qualitatively and quantitatively similar in six tubule segments containing no stellate cells; V_t decreased from 37.8±7.0 mV to 3.4±0.6 mV, R_t decreased from 8.8±2.1 kcm to 1.7±0.2 kcm, and DP_Cl increased from 5.8±2.6 mV to 50.0±2.1 mV. Thus, stellate cells are not required for signaling or mediating the effects of leucokinin in Malpighian tubules of Aedes aegypti. The results further support previous observations that principal cells signal the effects of leucokinin to increase the Cl^- conductance of the paracellular pathway through septate (or tight) junctions.

Key words: leucokinin, paracellular Cl^- conductance, tight junction, septate junction, Malpighian tubule, yellow fever mosquito, Aedes aegypti

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