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First published online December 3, 2004
Journal of Experimental Biology 207, 4663-4677 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01272
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Neuronal and neurohormonal control of the heart in the stomatopod crustacean, Squilla oratoria

Hiroshi Ando1 and Kiyoaki Kuwasawa2,*

1 Department of Oral Physiology, Matsumoto Dental University School of Dentistry, Shiojiri 399-0781, Japan
2 Neurobiology Laboratory, Faculty of Science, Okayama University of Science, Ridai-cho 1-1, Okayama 700-0005, Japan

* Author for correspondence (e-mail: kuwasawa-kiyoaki{at}das.ous.ac.jp)

Accepted 6 September 2004

The heart of Squilla oratoria contains a cardiac ganglion that consists of 15 intrinsic neurons, supplied by a pair of inhibitory nerves and two pairs of excitatory nerves, arising from the central nervous system. These comprise the extrinsic cardiac innervation. The paired cardio-inhibitor (CI) nerves run out in the 10th pair of nerve roots emerging from the subesophageal ganglion (SEG). The cell bodies of the CI neurons are found in the hemisphere of the 1st segment of the SEG contralateral to the nerve roots in which the CI axons emerge. The two pairs of 1st and 2nd cardio-accelerator (CA1 and CA2) nerves run out in the 16th and 19th pairs of nerve roots of the SEG. The cell bodies of the CA1 and CA2 neurons are found in the hemispheres of the 3rd and 4th segments of the SEG ipsilateral to the nerve roots in which the CA1 and CA2 axons are found.

The heartbeat was activated by application of glutamate, serotonin, dopamine, octopamine or acetylcholine, which were applied to the heart by perfusion into an organ bath. Joro-spider toxin (JSTX) blocked myocardial excitatory junctional potentials evoked by the cardiac ganglion. Neuronal cell bodies and processes in the heart were examined using immunocytochemical techniques. All 15 neurons of the cardiac ganglion showed glutamate-like immunoreactivity. Glutamate may be a neurotransmitter of the cardiac ganglion neurons.

JSTX also blocked cardiac acceleration by activation of CA1 and CA2 axons. CA1 and CA2 axons showed glutamate-like immunoreactivity. It is likely that glutamate is a neurotransmitter for the cardio-acceleratory neurons.

The heartbeat was inhibited by application of {gamma}-amino-butyric acid (GABA). Cardiac inhibition induced by activation of CI axons was blocked by picrotoxin. CI axons showed GABA-like immunoreactivity. These results may support the identification of GABA as an extrinsic inhibitory neurotransmitter.

Key words: cardioregulatory neuron, cardiac ganglion neuron, neurotransmitter, stomatopod, Squilla oratoria, immunocytochemistry, GABA, glutamate


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© The Company of Biologists Ltd 2004