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First published online November 19, 2004
Journal of Experimental Biology 207, 4439-4450 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01273

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Peptidergic innervation of the vasoconstrictor muscle of the abdominal aorta in Aplysia kurodai

Kosei Sasaki, Fumihiro Morishita and Yasuo Furukawa^*

Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan

^* Author for correspondence at present address: Laboratory of Neurobiology, Faculty of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima 739-8521, Japan (e-mail: yasfuru{at}hiroshima-u.ac.jp)

Accepted 6 September 2004

The arterial system of the marine mollusc Aplysia consists of three major arteries. One of them, the abdominal aorta, has a sphincter (the vasoconstrictor muscle) at the base of the artery. Contraction of this muscle reduces the blood flow into the abdominal aorta, thereby, playing a role in the regulation of the blood distribution in Aplysia. Here, we show the contractility of the vasoconstrictor muscle is modulated by three types of endogenous peptides, Aplysia mytilus inhibitory peptide-related peptides (AMRP), enterin and NdWFamide. Immunohistochemistry showed that putative neuronal processes containing the three peptides exist in the vasoconstrictor muscle. Enterin inhibited the muscle contraction elicited by the nerve stimulation or the application of a putative excitatory transmitter, acetylcholine (ACh). Enterin hyperpolarized the resting potential of the muscle and decreased the amplitude of the excitatory junction potential (EJP). AMRP also inhibited the nerve-evoked contraction although its action on the ACh-induced contraction was variable. AMRP also reduced the size of EJP, but had no effect on the resting potential of the muscle. NdWFamide enhanced the nerve-evoked contraction but not the ACh-induced contraction. NdWFamide augmented EJP without affecting the resting potential of the muscle. These results suggest that AMRP, enterin and NdWFamide are endogenous modulators of the contractile activity of the vasoconstrictor muscle, and that the peptidergic innervations of this muscle contribute to fine tuning of the blood distribution in Aplysia.

Key words: cardiovascular system, peptide, mollusc, Aplysia