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First published online September 15, 2004
Journal of Experimental Biology 207, 3731-3739 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01208

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The anterior stomach of larval mosquitoes (Aedes aegypti): effects of neuropeptides on transepithelial ion transport and muscular motility

H. Onken^*, S. B. Moffett and D. F. Moffett

School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA

^* Author for correspondence (e-mail: onkenh{at}wsu.edu)

Accepted 26 July 2004

The present investigation studied the influence of a number of neuropeptides on semi-open preparations of the isolated and perfused anterior stomach of larval Aedes aegypti. Effects of peptides were observed on the lumen negative transepithelial voltage (V_te) that is present with serotonin in the bath; this voltage most likely reflects active HCO₃^– secretion involved in alkalization of the larval anterior stomach. The five different A. aegypti allatostatins (allatostatin A 1–5) all affected V_te in almost identical ways, causing a 10–15% reduction of the voltage at 10^–7 mol l^–1. A. aegypti neuropeptide F and proctolin reduced V_te at submicromolar concentrations. At 10^–6 mol l^–1, neuropeptide F reduced V_te by 30% and proctolin reduced V_te by 50%. In contrast, A. aegypti allatotropin, A. aegypti head peptides I and III and A. aegypti short neuropeptide F were without effect on V_te. During the investigation it was observed that the peristaltic contractions of the preparations caused a dynamic component of V_te. Peristaltic contractions and the correlated voltage fluctuations depended on the presence of serotonin. Peristaltic activity and V_te deflections were progressively inhibited by A. aegypti head peptides I and III by A. aegypti short neuropeptide F and by A. aegypti neuropeptide F when the peptide concentrations were increased from 10^–8 to 10^–6 mol l^–1. These observations show that physiological concentrations of some of the tested neuropeptides affect two processes that require coordination: ion transport and motility of the larval anterior stomach.

Key words: Aedes aegypti, allatostatin, allatotropin, anterior stomach, head peptide, larva, midgut, mosquito, neuropeptide F, peristalsis, proctolin, transepithelial voltage

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