QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online May 24, 2004
Journal of Experimental Biology 207, 2229-2236 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01007

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ocalewicz, K. Articles by Goryczko, K. Search for Related Content PubMed PubMed Citation Articles by Ocalewicz, K. Articles by Goryczko, K.

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

K. Ocalewicz^1,*, I. Babiak^2,3, S. Dobosz⁴, J. Nowaczyk¹ and K. Goryczko⁵

¹ Department of Evolutionary Genetics, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
² Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
³ Department of Fisheries and Natural Sciences, Bodø Regional University, 8049 Bodø, Norway
⁴ Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, 83-330 Zukowo, Poland
⁵ Department of Fish Biology and Farming, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

^* Author for correspondence at present address: Laboratoire de Genetique des Poissons, Institut National de la Recherche Agronomique, Centre de Recherche de Jouy-en-Josas, Domaine de Vilvert, F-78352 Jouy-en-Josas Cedex, France (e-mail: kocale{at}diamant.jouy.inra.fr)

Accepted 1 April 2004

The study provides new data on the stability of gamma radiation-induced chromosome fragments of a putative maternal nuclear genome in an androgenetic vertebrate, rainbow trout (Oncorhynchus mykiss Walbaum). The fragments were found in five of 16 examined individuals and they were mostly centromeric parts of metacentric or subtelocentric chromosomes. Chromosome fragments were identical in all cells of a given androgenetic individual, indicating that segregation of chromosome fragments is active from the early cell divisions. Most of the fragments were telomereless, i.e. they had no telomeric sequences on their ends. This shows that telomeres are not necessary for stability of chromosomal structures in a vertebrate genome. In one individual, the interstitial telomeric sites were found in chromosomes, which could be the effect of joining chromosome fragments.

Key words: androgenesis, centromere, chromosome, fish, gamma radiation, telomere