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First published online April 23, 2004
Journal of Experimental Biology 207, 1915-1924 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00975

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Force development, energy state and ATP production of cardiac muscle from turtles and trout during normoxia and severe hypoxia

Johannes Overgaard and Hans Gesser^*

Department of Zoophysiology, Institute of Biological Sciences, University of Aarhus, DK 8000 Aarhus, Denmark

^* Author for correspondence (e-mail: hans.gesser{at}biology.au.dk)

Accepted 10 March 2004

The effects of hypoxia on energy economy of cardiac muscle were compared between the hypoxia-tolerant freshwater turtle at 20°C and the hypoxia-sensitive rainbow trout at 15°C. Isolated ventricular preparations were left either at rest or stimulated at 30 min^-1 to develop isometric twitch force. Under oxygenated conditions, twitch force and oxygen consumption were similar for the two species. Overall metabolism was reduced during severe hypoxia in both resting and stimulated preparations and under these conditions most of the ATP production was derived from anaerobic metabolism. During hypoxia, a metabolic depression of approximately 2/3 occurred for non-contractile processes in both turtle and trout preparations. During hypoxia, lactate production and residual oxygen consumption were similar in turtle and trout. Cellular energy state and phosphorylation potential decreased during severe hypoxia in both species and this reduction was more severe in preparations stimulated to contraction. However, in turtle ventricular preparations the energy state and phosphorylation potential stabilised at higher levels than in trout, and turtle preparations also maintained a higher twitch force throughout the hypoxic period. Moreover, twitch force relative to total ATP hydrolysis was markedly increased during hypoxia in turtle while this ratio was unchanged for trout. The main findings of this study are: (1) cellular energy liberation and the energy demand of non-contractile processes decreased to similar levels in hypoxic turtle and trout myocardium; (2) turtle myocardium maintained a substantially higher cellular energy state and twitch force development than trout myocardium during hypoxia and (3) the ratio of twitch force to ATP hydrolysis increased during hypoxia in turtle but was unchanged in trout. It is possible that this superior economy of the contracting turtle myocardium contributes to the remarkable hypoxia tolerance of freshwater turtles.

Key words: oxygen consumption, lactate production, cellular energy state, phosphorylation potential, cost of contraction, twitch force, metabolic depression, Trachemys scripta, Oncorhynchus mykiss

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