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The Journal of Experimental Biology 206, 1319-1323 (2003)
doi: 10.1242/jeb.00266

Prolactin increases open-channel density of epithelial Na+ channel in adult frog skin

Makoto Takada* and Miyoko Kasai

Department of Physiology, Saitama Medical School, Moroyama, Iruma-gun, Saitama, 3500495 Japan

* Author for correspondence (e-mail: makokam{at}saitama-med.ac.jp)

Accepted 28 January 2003

The short-term effect of prolactin on the skin of the adult tree frog Hyla arborea japonica was investigated using current-fluctuation analysis. Basolateral application of ovine prolactin (10 µg ml-1) (1) increased the amiloride-blockable short-circuit current (SCC) across the skin 2.6±0.4-fold and (2) increased the open-channel density (M) of the epithelial Na+ channel 6.1±1.2-fold but decreased the single-channel current i to 0.4±0.1 times the control value (N=9). The increase in SCC induced by prolactin was thus due to an increase in M, not i. Apparently, in amphibians prolactin has not only a counteracting effect on metamorphosis but also a stimulatory effect on the development of adult-type features, such as this amiloride-blockable SCC.

Key words: frog skin, current-fluctuation (noise) analysis, prolactin, active Na+ transport, epithelial Na+ channel (ENaC), tree frog, Hyla arborea japonica


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