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The Journal of Experimental Biology 206, 959-965 (2003)
doi: 10.1242/jeb.00201

K+ transport in Malpighian tubules of Tenebrio molitor L.: is a KATP channel involved?

U. I. M. Wiehart1, G. Klein2, P. Steels2, S. W. Nicolson1 and E. Van Kerkhove2,*

1 Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa
2 Laboratory of Physiology, Biomed CMK, Limburgs Universitair Centrum, B3590 Diepenbeek, Belgium

* Author for correspondence (e-mail: emmy.vankerkhove{at}luc.ac.be)

Accepted 10 December 2002

The presence of ATP-regulated K+ (KATP) channels in Tenebrio molitor Malpighian tubules was investigated by examining the effect of glibenclamide on both fluid secretion and basolateral membrane potentials (Vbl). Glibenclamide, a KATP channel blocker, slowed fluid secretion of Tenebrio tubules. In low bath K+ concentration (5 mmol l-1), glibenclamide either hyperpolarized or depolarized Vbl, resembling the effect seen with Ba2+. Subsequent addition of 6 mmol l-1 Ba2+ caused a further hyper- or depolarization of Vbl. In control Ringer (50 mmol l-1 KCl, 90 mmol l-1 NaCl), glibenclamide had no visible effect on Vbl. The effect of ouabain was investigated in low bath [K+] in the presence of Ba2+. Vbl responded by a small but significant hyperpolarization from -51±4 mV to -56±4 mV (n=16, P<0.001) in response to 1 mmol l-1 ouabain. Repeating the experiments in the presence of both glibenclamide and Ba2+ resulted in a depolarization of Vbl when ouabain was added. In low bath [K+] (high Na+), the Na+/K+-ATPase is expected to function at a high rate. In the presence of Ba2+, replacing Na+ by K+ rapidly depolarized Vbl, but this was followed by a repolarization. Repeating the experiments in the presence of glibenclamide markedly reduced the depolarizing effect and abolished the repolarization, with a gradual decrease in the sensitivity of Vbl to the surrounding [K+]. These results suggest the presence of KATP channels in the basolateral membrane. Glibenclamide had no visible effect on Vbl in high K+ or in the absence of Ba2+, indicating that other highly conductive K+ channels may mask the effect on KATP channels. This is the first demonstration of the presence of KATP channels in an insect epithelium.

Key words: K+ transport, KATP channel, Malpighian tubules, Tenebrio molitor, glibenclamide, basolateral membrane potential, fluid secretion rate


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