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The Journal of Experimental Biology 206, 1051-1057 (2003)
doi: 10.1242/jeb.00216

Acutely altered hemodynamics following venous obstruction in the early chick embryo

Sandra Stekelenburg-de Vos¹, Nicolette T. C. Ursem^1,*, Wim C. J. Hop², Juriy W. Wladimiroff¹, Adriana C. Gittenberger-de Groot³ and Robert E. Poelmann³

¹ Department of Obstetrics and Gynaecology, Erasmus MC, Rotterdam, The Netherlands
² Department of Epidemiology and Biostatistics, Erasmus MC, Rotterdam, The Netherlands
³ Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands

^* Author for correspondence (e-mail: n.ursem{at}erasmusmc.nl)

Accepted 10 January 2003

In the venous clip model specific cardiac malformations are induced in the chick embryo by obstructing the right lateral vitelline vein with a microclip. Clipping alters venous return and intracardiac laminar blood flow patterns, with secondary effects on the mechanical load of the embryonic myocardium. We investigated the instantaneous effects of clipping the right lateral vitelline vein on hemodynamics in the stage-17 chick embryo. 32 chick embryos HH 17 were subdivided into venous clipped (N=16) and matched control embryos (N=16). Dorsal aortic blood flow velocity was measured with a 20 MHz pulsed Doppler meter. A time series of eight successive measurements per embryo was made starting just before clipping and ending 5h after clipping. Heart rate, peak systolic velocity, time-averaged velocity, peak blood flow, mean blood flow, peak acceleration and stroke volume were determined. All hemodynamic parameters decreased acutely after venous clipping and only three out of seven parameters (heart rate, time-averaged velocity and mean blood flow) showed a recovery to baseline values during the 5h study period. We conclude that the experimental alteration of venous return has major acute effects on hemodynamics in the chick embryo. These effects may be responsible for the observed cardiac malformations after clipping.

Key words: chick embryo, Doppler ultrasound, hemodynamics, blood flow velocity, venous clip model

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