QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Skov, P. V. Articles by Steffensen, J. F. Search for Related Content PubMed PubMed Citation Articles by Skov, P. V. Articles by Steffensen, J. F. Social Bookmarking                         What's this?

The Journal of Experimental Biology 206, 591-599 (2003)
doi: 10.1242/jeb.00113

The blood volumes of the primary and secondary circulatory system in the Atlantic cod Gadus morhua L., using plasma bound Evans Blue and compartmental analysis

P. V. Skov^* and J. F. Steffensen

Marine Biological Laboratory, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør, Denmark

^* Author for correspondence at present address: School of Biomedical Sciences, Department of Anatomy and Developmental Biology, University of Queensland, St Lucia, Queensland 4072, Australia (e-mail: peter.skov{at}mailbox.uq.edu.au)

Accepted 28 October 2002

The volume of the primary (PCS) and secondary (SCS) circulatory system in the Atlantic cod Gadus morhua was determined using a modified dye dilution technique. Cod (N=10) were chronically cannulated in the second afferent branchial artery with PE-50 tubing. Evans Blue dye was bound to harvested fish plasma at a concentration of 1 mg dye ml^-1 plasma, and injected at a concentration of 1 mg kg^-1 body mass. Serial sampling from the cannula produced a dye dilution curve, which could be described by a double exponential decay equation. Curve analysis enabled the calculation of the primary circulatory and total distribution volume. The difference between these volumes is assumed to be the volume of the SCS. From the dilution curve, it was also possible to calculate flow rates between and within the systems. The results of these experiments suggest a plasma volume in the PCS of 3.42±0.89 ml 100 g^-1 body mass, and in the SCS of 1.68±0.35 ml 100 g^-1 body mass (mean ± S.D.) or approximately 50% that of the PCS. Flow rates to the SCS were calculated as 2.7% of the resting cardiac output. There was an allometric relationship between body mass and blood volumes. Increasing condition factor showed a tendency towards smaller blood volumes of the PCS, expressed as percentage body mass, but this was not evident for the volume of the SCS.

Key words: secondary circulation, volume, flow, leak, Evans Blue, plasma-bound tracer, Atlantic cod, Gadus morhua

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Seternes, T. C. Tonheim, M. Lovoll, J. Bogwald, and R. A. Dalmo Specific endocytosis and degradation of naked DNA in the endocardial cells of cod (Gadus morhua L.) J. Exp. Biol., June 15, 2007; 210(12): 2091 - 2103. [Abstract] [Full Text] [PDF]