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First published online November 10, 2003
Na+/K+-ATPase 
-isoform switching in gills of rainbow trout (Oncorhynchus mykiss) during salinity transfer
1 Department of Zoology, The University of British Columbia, Vancouver,
British Columbia, Canada, V6T 1Z4
2 Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
N2L 3G1
3 Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G
2W1
Author for correspondence (e-mail:
jrichard{at}zoology.ubc.ca)
Accepted 2 September 2003
We identified five Na+/K+-ATPase 
-isoforms in
rainbow trout and characterized their expression pattern in gills following
seawater transfer. Three of these isoforms were closely related to other
vertebrate 1 isoforms (designated 1a, 1b and 1c),
one isoform was closely related to 2 isoforms (designated 2) and
the fifth was closely related to 3 isoforms (designated 3).
Na+/K+-ATPase 1c- and 3-isoforms were
present in all tissues examined, while all others had tissue specific
distributions. Four Na+/K+-ATPase -isoforms were
expressed in trout gills (1a, 1b, 1c and 3).
Na+/K+-ATPase 1c- and 3-isoforms were
expressed at low levels in freshwater trout gills and their expression pattern
did not change following transfer to 40% or 80% seawater.
Na+/K+-ATPase 1a and 1b were
differentially expressed following seawater transfer. Transfer from freshwater
to 40% and 80% seawater decreased gill Na+/K+-ATPase
1a mRNA, while transfer from freshwater to 80% seawater caused a
transient increase in Na+/K+-ATPase 1b mRNA.
These changes in isoform distribution were accompanied by an increase in gill
Na+/K+-ATPase enzyme activity by 10 days after transfer
to 80% seawater, though no significant change occurred following transfer to
40% seawater. Isoform switching in trout gills following salinity transfer
suggests that the Na+/K+-ATPase 1a- and
1b-isoforms play different roles in freshwater and seawater
acclimation, and that assays of Na+/K+-ATPase enzyme
activity may not provide a complete picture of the role of this protein in
seawater transfer.
Key words: gene expression, seawater, freshwater, ion regulation, salmonid, rainbow trout, Oncorhynchus mykiss, Na+/K+-ATPase
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