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The Journal of Experimental Biology 205, 719-744 (2002)
© 2002 The Company of Biologists Limited

A comparative study of odorant binding protein genes: differential expression of the PBP1-GOBP2 gene cluster in Manduca sexta (Lepidoptera) and the organization of OBP genes in Drosophila melanogaster (Diptera)

Richard G. Vogt*, Matthew E. Rogers, Marie-dominique Franco{ddagger} and Ming Sun

Department of Biological Sciences, University of South Carolina, Columbia, SC 29208 USA
Present address: Department of Biological Sciences, Columbia University, New York, NY 10027, USA
{ddagger} Present address: Department of Biology, Regis College, 3333 Regis Boulevard, Denver, CO 80221, USA

*Author for correspondence (e-mail: vogt{at}biol.sc.edu)

Accepted 10 December 2001

Insects discriminate odors using sensory organs called olfactory sensilla, which display a wide range of phenotypes. Sensilla express ensembles of proteins, including odorant binding proteins (OBPs), olfactory receptors (ORs) and odor degrading enzymes (ODEs); odors are thought to be transported to ORs by OBPs and subsequently degraded by ODEs. These proteins belong to multigene families. The unique combinatorial expression of specific members of each of these gene families determines, in part, the phenotype of a sensillum and what odors it can detect. Furthermore, OBPs, ORs and ODEs are expressed in different cell types, suggesting the need for cell–cell communication to coordinate their expression. This report examines the OBP gene family. In Manduca sexta, the genes encoding PBP1Msex and GOBP2Msex are sequenced, shown to be adjacent to one another, and characterized together with OBP gene structures of other lepidoptera and Drosophila melanogaster. Expression of PBP1Msex, GOBP1Msex and GOBP2Msex is characterized in adult male and female antenna and in larval antenna and maxilla. The genomic organization of 25 D. melanogaster OBPs are characterized with respect to gene locus, gene cluster, amino acid sequence similarity, exon conservation and proximity to OR loci, and their sequences are compared with 14 M. sexta OBPs. Sensilla serve as portals of important behavioral information, and genes supporting sensilla function are presumably under significant evolutionary selective pressures. This study provides a basis for studying the evolution of the OBP gene family, the regulatory mechanisms governing the coordinated expression of OBPs, ORs and ODEs, and the processes that determine specific sensillum phenotypes.

Key words: Manduca sexta, Drosophila melanogaster, odorant binding protein, olfactory receptor, odor degrading enzyme, gene expression, olfactory sensilla, olfaction.


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© The Company of Biologists Ltd 2002