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The Journal of Experimental Biology 205, 71-77 (2002)
© 2002 The Company of Biologists Limited

Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity

C. M. Luquet1,*, U. Postel2, J. Halperin1,3, M. R. Urcola1, R. Marques4 and D. Siebers2

1 Department of Biological Sciences, FCEN University of Buenos Aires, Building II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina,
2 Alfred Wegener Institute for Polar and Marine Research, Columbusstrasse, D-27568 Bremerhaven, Germany,
3 CONICET-Argentina and
4 Department of Inorganic Chemistry, FCEN University of Buenos Aires, Argentina

*e-mail: luquet{at}bg.fcen.uba.ar

Accepted 1 October 2001

We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6–8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions.

The gills of crabs acclimated to low salinity, perfused and bathed with 10 {per thousand} saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, –10.2±1.6, –10.8±1.3 and –6.7±1.3 mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30 {per thousand} to 20 {per thousand} or 10 {per thousand} increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5 % and 60±5 % after perfusion with ouabain or BaCl2 (5 mmol l–1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 µequiv g–1 h–1.

Gill 6 of crabs acclimated to high salinity produced TEPDs of –1.5±0.1 and –1.3±0.09 mV after perfusion with 30 {per thousand} or 40 {per thousand} salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7 % and 86±4 %, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 µequiv g–1 h–1 was recorded in gill 6 perfused with 38 {per thousand} saline.

Key words: isolated perfused gill, transepithelial potential difference, Na+K+-ATPase, ion flux, hypo-regulation, hyper-regulation, ouabain, crab, Chasmagnathus granulatus.


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