|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 204, Issue 9 1659-1666, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
JD Kelty and RE Lee Jr
Department of Zoology, 212 Pearson Hall, Miami University, Oxford, OH 45056, USA. j-kelty@uchicago.edu
In contrast to most studies of rapid cold-hardening, in which abrupt transfers to low temperatures are used to induce an acclimatory response, the primary objectives of this study were to determine (i) whether rapid cold-hardening was induced during the cooling phase of an ecologically based thermoperiod, (ii) whether the protection afforded was lost during warming or contributed to increased cold-tolerance during subsequent cycles and (iii) whether the major thermally inducible stress protein (Hsp70) or carbohydrate cryoprotectants contributed to the protection afforded by rapid cold-hardening. During the cooling phase of a single ecologically based thermoperiod, the tolerance of Drosophila melanogaster to 1 h at -7 degrees C increased from 5 +/- 5% survival to 62.5 +/- 7.3% (means +/- S.E.M., N=40-60), while their critical thermal minima (CTmin) decreased by 1.9 degrees C. Cold hardiness increased with the number of thermoperiods to which flies were exposed; i.e. flies exposed to six thermoperiods were more cold-tolerant than those exposed to two. Endogenous levels of Hsp70 and carbohydrate cryoprotectants were unchanged in rapidly cold-hardened adults compared with controls held at a constant 23 degrees C. In nature, rapid cold-hardening probably affords subtle benefits during short-term cooling, such as allowing D. melanogaster to remain active at lower temperatures than they otherwise could.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  N. M. Teets, M. A. Elnitsky, J. B. Benoit, G. Lopez-Martinez, D. L. Denlinger, and R. E. Lee Jr. Rapid cold-hardening in larvae of the Antarctic midge Belgica antarctica: cellular cold-sensing and a role for calcium Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1938 - R1946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S Terblanche, J. A Deere, S. Clusella-Trullas, C. Janion, and S. L Chown Critical thermal limits depend on methodological context Proc R Soc B, December 7, 2007; 274(1628): 2935 - 2943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fujiwara and D. L. Denlinger p38 MAPK is a likely component of the signal transduction pathway triggering rapid cold hardening in the flesh fly Sarcophaga crassipalpis J. Exp. Biol., September 15, 2007; 210(18): 3295 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Lee Jr, M. A. Elnitsky, J. P. Rinehart, S. A. L. Hayward, L. H. Sandro, and D. L. Denlinger Rapid cold-hardening increases the freezing tolerance of the Antarctic midge Belgica antarctica J. Exp. Biol., February 1, 2006; 209(3): 399 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Shreve, J. D. Kelty, and R. E. Lee Jr Preservation of reproductive behaviors during modest cooling: rapid cold-hardening fine-tunes organismal response J. Exp. Biol., May 1, 2004; 207(11): 1797 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Sinclair, C. J. Klok, and S. L. Chown Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing J. Exp. Biol., March 15, 2004; 207(8): 1287 - 1294. [Abstract] [Full Text] [PDF]
|
|
