QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Movies References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Noguchi, M. Articles by Akazawa, T. Search for Related Content PubMed PubMed Citation Articles by Noguchi, M. Articles by Akazawa, T. Social Bookmarking                         What's this?

Journal of Experimental Biology, Vol 204, Issue 6 1063-1071, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

ATP-regenerating system in the cilia of Paramecium caudatum

M Noguchi, T Sawada and T Akazawa
Department of Environmental Biology and Chemistry, Faculty of Science, Toyama University, Toyama 930-8555, Japan. noguchi@sci.toyama-u.ac.jp

The energy supply for eukaryotic ciliary and flagellar movement is thought to be maintained by ATP-regenerating enzymes such as adenylate kinase, creatine kinase and arginine kinase. In this study, the energy-supplying system for the ciliary movement of Paramecium caudatum was examined. Arginine kinase and adenylate kinase activities were detected in the cilia. To demonstrate that phosphoarginine satisfactorily supplies high-energy phosphate compounds into the narrow ciliary space, we prepared an intact ciliated cortical sheet from live Paramecium caudatum. These cortical sheets, with an intact ciliary membrane, produced a half-closed system in which each cilium was covered with a ciliary membrane with an opening to the cell body. Ciliary beating on the intact cortical sheets was induced by perfusing not only ATP but also ADP. Addition of phosphoarginine (0.2 mmol l(-1)) increased the beat frequency. A further increase in beat frequency was observed in 0.4 mmol l(-1) phosphoarginine, and this was enhanced when the cilia were reactivated with relatively low concentrations of ATP. We have demonstrated that phosphoarginine supplies energy as a 'phosphagen' for ciliary beating in Paramecium caudatum, suggesting that phosphoarginine functions not only as a reservoir of energy but also as a transporter of energy in these continuously energy-consuming circumstances. http://www.biologists.com/JEB/movies/jeb3123.html
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				B. F. Mitchell, L. B. Pedersen, M. Feely, J. L. Rosenbaum, and D. R. Mitchell ATP Production in Chlamydomonas reinhardtii Flagella by Glycolytic Enzymes Mol. Biol. Cell, October 1, 2005; 16(10): 4509 - 4518. [Abstract] [Full Text] [PDF]

				S. Perovic-Ottstadt, M. Wiens, H.-C. Schroder, R. Batel, M. Giovine, A. Krasko, I. M. Muller, and W. E. G. Muller Arginine kinase in the demosponge Suberites domuncula: regulation of its expression and catalytic activity by silicic acid J. Exp. Biol., February 15, 2005; 208(4): 637 - 646. [Abstract] [Full Text] [PDF]

				H. Zhang and D. R. Mitchell Cpc1, a Chlamydomonas central pair protein with an adenylate kinase domain J. Cell Sci., August 15, 2004; 117(18): 4179 - 4188. [Abstract] [Full Text] [PDF]

				M. Wirschell, G. Pazour, A. Yoda, M. Hirono, R. Kamiya, and G. B. Witman Oda5p, a Novel Axonemal Protein Required for Assembly of the Outer Dynein Arm and an Associated Adenylate Kinase Mol. Biol. Cell, June 1, 2004; 15(6): 2729 - 2741. [Abstract] [Full Text] [PDF]

				F. Canonaco, U. Schlattner, P. S. Pruett, T. Wallimann, and U. Sauer Functional Expression of Phosphagen Kinase Systems Confers Resistance to Transient Stresses in Saccharomyces cerevisiae by Buffering the ATP Pool J. Biol. Chem., August 23, 2002; 277(35): 31303 - 31309. [Abstract] [Full Text] [PDF]