|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Chemolithoheterotrophy in a metazoan tissue: thiosulfate production matches ATP demand in ciliated mussel gills
1 Department of Biology, University of Alabama at Birmingham, 1300 University Boulevard, Birmingham, AL 35294-1170, USA and
2 Institut für Zoophysiologie, Heinrich-Heine-Universität, Universitätsstraße 1, 40225 Düsseldorf, Germany
*e-mail: doeller{at}uab.edu
Accepted August 10, 2001
The ribbed mussel Geukensia demissa inhabits sulfide-rich coastal sediments with a distribution that suggests a preference for exposure to sulfide. Although sulfide is a respiratory poison, it is also a potent reductant. Geukensia demissa gill mitochondria can use sulfide as a respiratory substrate for ATP production, and the gills of this species exhibit sulfide-supported oxygen consumption that matches the energy demand of ciliary beating. Here, we demonstrate (i) that the major product of G. demissa gill sulfide oxidation is thiosulfate and (ii) that the rate of sulfide oxidation also matches the cellular energy demand, resulting in a ratio near unity of oxygen consumed to sulfide oxidized at both low and high ciliary beat frequencies. A value for this ratio of unity is consistent with electrons from sulfide oxidation entering the mitochondrial electron transport chain. In the gills of the blue mussel Mytilus edulis from sulfide-free conditions, this ratio is 3–5 times higher, indicating an uncoupling of oxygen consumption from sulfide oxidation. Whereas M. edulis gills exhibit anaerobic metabolism during sulfide exposure, G. demissa gills do not, indicating a difference in sulfide tolerance between the two mussel species.
Key words: sulphide, gills, sulphide oxidation, bromobimane HPLC, succinate, mussel, Geukensia demissa, Mytilus edulis.
This article has been cited by other articles:
|
|
 |
|
  M. Goubern, M. Andriamihaja, T. Nubel, F. Blachier, and F. Bouillaud Sulfide, the first inorganic substrate for human cells FASEB J, June 1, 2007; 21(8): 1699 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Koenitzer, T. S. Isbell, H. D. Patel, G. A. Benavides, D. A. Dickinson, R. P. Patel, V. M. Darley-Usmar, J. R. Lancaster Jr, J. E. Doeller, and D. W. Kraus Hydrogen sulfide mediates vasoactivity in an O2-dependent manner Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1953 - H1960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Gainey Jr. and M. J. Greenberg Hydrogen Sulfide Is Synthesized in the Gills of the Clam Mercenaria mercenaria and Acts Seasonally to Modulate Branchial Muscle Contraction Biol. Bull., August 1, 2005; 209(1): 11 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Kraus and J. E. Doeller Sulfide consumption by mussel gill mitochondria is not strictly tied to oxygen reduction: measurements using a novel polarographic sulfide sensor J. Exp. Biol., October 1, 2004; 207(21): 3667 - 3679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoffmeister, A. van der Klei, C. Rotte, K. W. A. van Grinsven, J. J. van Hellemond, K. Henze, A. G. M. Tielens, and W. Martin Euglena gracilis Rhodoquinone:Ubiquinone Ratio and Mitochondrial Proteome Differ under Aerobic and Anaerobic Conditions J. Biol. Chem., May 21, 2004; 279(21): 22422 - 22429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Theissen, M. Hoffmeister, M. Grieshaber, and W. Martin Single Eubacterial Origin of Eukaryotic Sulfide:Quinone Oxidoreductase, a Mitochondrial Enzyme Conserved from the Early Evolution of Eukaryotes During Anoxic and Sulfidic Times Mol. Biol. Evol., September 1, 2003; 20(9): 1564 - 1574. [Abstract] [Full Text] [PDF]
|
|
