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The Journal of Experimental Biology 204, 2763-2771 (2001)
© 2001 The Company of Biologists Limited

TEMPERATURE AND MYOGENIC FACTOR TRANSCRIPT LEVELS DURING EARLY DEVELOPMENT DETERMINES MUSCLE GROWTH POTENTIAL IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) AND SEA BASS (DICENTRARCHUS LABRAX)

D. WILKES¹, S. Q. XIE², N. C. STICKLAND², H. ALAMI-DURANTE³, M. KENTOURI⁴, A. STERIOTI⁵, G. KOUMOUNDOUROS⁵, B. FAUCONNEAU⁶ and G. GOLDSPINK^1,*

¹ Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, University of London, Rowland Hill Street, London NW3 2PF, UK
² Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
³ INRA-IFREMER, Station D Hydrobiologie 64310 Saint Pée Sur Nivelle, France
⁴ Biology Department, University of Crete, PO Box 1470, 71110 Iraklio, Crete, Greece
⁵ Institute of Marine Biology of Crete, PO Box 2214, 71003 Iraklio, Crete, Greece
⁶ INRA Fish Physiology, Campus de Beaulieu, 35042 Rennes, France
^* Author for correspondence (e-mail: g.goldspink{at}rfc.ucl.ac.uk )

Accepted May 17, 2001

The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development.

Key words: Muscle development, trout, sea bass, growth, MyoD, myogenin, myosin, MRF, rainbow trout, Oncorhynchus mykiss, sea bass, Dicentrarchus labrax

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