QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Teerijoki, H. Articles by Mölsä, H. Search for Related Content PubMed PubMed Citation Articles by Teerijoki, H. Articles by Mölsä, H.

The Journal of Experimental Biology 204, 2667-2673 (2001)
© 2001 The Company of Biologists Limited

Rainbow trout glucose transporter (OnmyGLUT1): functional assessment in Xenopus laevis oocytes and expression in fish embryos

Heli Teerijoki¹, Aleksei Krasnov^1,*, Yuri Gorodilov², Sanjeev Krishna³ and Hannu Mölsä¹

¹ Institute of Applied Biotechnology, University of Kuopio, PO Box 1627, Kuopio 70211, Finland,
² Biological Institute, University of Sanct Petersburg, Oranienbaum Chaussee 2, Stary Peterhof, Sanct Petersburg 198504, Russia and
³ Department of Infectious Diseases, St George’s Hospital Medical School, London SW17 0RE, UK

*Author for correspondence (e-mail: krasnov{at}uku.fi)

Accepted May 25, 2001

Recently, we reported the cloning of a putative glucose transporter (OnmyGLUT1) from rainbow trout embryos. In this paper, we describe the functional characteristics of OnmyGLUT1 and its expression during embryonic development of rainbow trout. Transport of D-glucose was analysed in Xenopus laevis oocytes following microinjection of mRNA transcribed in vitro. These experiments confirmed that OnmyGLUT1 is a facilitative Na⁺-independent transporter. Assessment of substrate selectivity, sensitivity to cytochalasin B and phloretin and kinetic parameters showed that the rainbow trout glucose transporter was similar to a carp transporter and to mammalian GLUT1. Embryonic expression of OnmyGLUT1 was studied using whole-mount in situ hybridization. Ubiquitous distribution of transcripts was observed until the early phase of somitogenesis. During the course of organogenesis, somitic expression decreased along the rostro-caudal axis, finally ceasing in the mature somites. The OnmyGLUT1 transcripts were detected in the neural crest during the whole study period. Transcripts were also found in structures that are likely to originate from the neural crest cells (gill arches, pectoral fins, upper jaw, olfactory organs and primordia of mouth lips). Hexose transport activity was detected at all developmental stages after blastulation. Cytochalasin B blocked the accumulation of phosphorylated 2-deoxy-D-glucose by dissociated embryonic cells, suggesting an important role for transport in glucose metabolism.

Key words: glucose transporter, GLUT1, Xenopus laevis oocyte, development, expression, rainbow trout, Oncorhynchus mykiss, in situ hybridization, kinetics.

This article has been cited by other articles:

				P. K. Battiprolu, K. J. Harmon, and K. J. Rodnick Sex differences in energy metabolism and performance of teleost cardiac tissue Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R827 - R836. [Abstract] [Full Text] [PDF]

				J. R. Hall, C. E. Short, and W. R. Driedzic Sequence of Atlantic cod (Gadus morhua) GLUT4, GLUT2 and GPDH: developmental stage expression, tissue expression and relationship to starvation-induced changes in blood glucose J. Exp. Biol., November 15, 2006; 209(22): 4490 - 4502. [Abstract] [Full Text] [PDF]

				J. C. Kam and C. L. Milligan Fuel use during glycogenesis in rainbow trout (Oncorhynchus mykiss Walbaum) white muscle studied in vitro J. Exp. Biol., March 1, 2006; 209(5): 871 - 880. [Abstract] [Full Text] [PDF]

				J. R. Hall, T. J. MacCormack, C. A. Barry, and W. R. Driedzic Sequence and expression of a constitutive, facilitated glucose transporter (GLUT1) in Atlantic cod Gadus morhua J. Exp. Biol., December 15, 2004; 207(26): 4697 - 4706. [Abstract] [Full Text] [PDF]

				J. Frolow and C. L. Milligan Hormonal regulation of glycogen metabolism in white muscle slices from rainbow trout (Oncorhynchus mykiss Walbaum) Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1344 - R1353. [Abstract] [Full Text] [PDF]

				E. Capilla, M. Diaz, A. Albalat, I. Navarro, J. E. Pessin, K. Keller, and J. V. Planas Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E348 - E357. [Abstract] [Full Text] [PDF]

				C. L. Milligan A regulatory role for cortisol in muscle glycogen metabolism in rainbow trout Oncorhynchus mykiss Walbaum J. Exp. Biol., September 15, 2003; 206(18): 3167 - 3173. [Abstract] [Full Text] [PDF]

				A. Krasnov, H. Teerijoki, Y. Gorodilov, and H. Molsa Cloning of rainbow trout (Oncorhynchus mykiss) {alpha}-actin, myosin regulatory light chain genes and the 5'-flanking region of {alpha}-tropomyosin. Functional assessment of promoters J. Exp. Biol., February 1, 2003; 206(3): 601 - 608. [Abstract] [Full Text] [PDF]

				E. Capilla, M. Diaz, J. Gutierrez, and J. V. Planas Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E44 - E49. [Abstract] [Full Text] [PDF]