|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 203, Issue 8 1373-1381, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
H Onken, A Schobel, J Kraft and M Putzenlechner
Institut fur Tierphysiologie, Freie Universitat Berlin, Grunewaldstrasse 34, D-12165 Berlin, Germany. onkenh@ffclrp.usp.br
Split lamellae of the posterior gills of freshwater-adapted Chinese crabs (Eriocheir sinensis) were mounted in a modified Ussing-type chamber, and active and electrogenic absorption of Na(+) and Cl(-) were measured as positive (I(Na)) or negative (I(Cl)) short-circuit currents. Haemolymph-side addition of eyestalk extract stimulated I(Cl) by increasing both the transcellular Cl(-) conductance and the electromotive force for Cl(-) absorption. The effect was dose-dependent. Boiling the eyestalk extract did not change its effectiveness. The stimulating factor passed through dialysis tubing, indicating that it has a molecular mass of less than 2 kDa. R(p)cAMPS, a blocker of protein kinase A, reduced the stimulated I(Cl). Eyestalk extract stimulated I(Na) by increasing the transcellular Na(+) conductance at constant electromotive force. Amiloride-induced current-noise analysis revealed that stimulation of I(Na) was accompanied by an increase in the apparent number of open apical Na(+) channels at a slightly reduced single-channel current. In addition to the electrophysiological experiments, whole gills were perfused in the presence and in the absence of putative transport stimulators, and the specific activities of the V-ATPase and the Na(+)/K(+)-ATPase were measured. Eyestalk extract, theophylline or dibutyryl-cyclic AMP stimulated the activity of the V-ATPase, whereas the activity of the Na(+)/K(+)-ATPase was unaffected. The simultaneous presence of R(p)cAMPS prevented the stimulation of V-ATPase by eyestalk extract or theophylline.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Rein, M. Voss, W. Blenau, B. Walz, and O. Baumann Hormone-induced assembly and activation of V-ATPase in blowfly salivary glands is mediated by protein kinase A Am J Physiol Cell Physiol, January 1, 2008; 294(1): C56 - C65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Voss, O. Vitavska, B. Walz, H. Wieczorek, and O. Baumann Stimulus-induced Phosphorylation of Vacuolar H+-ATPase by Protein Kinase A J. Biol. Chem., November 16, 2007; 282(46): 33735 - 33742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Genovese, M. Senek, N. Ortiz, M. Regueira, D. W. Towle, M. Tresguerres, and C. M. Luquet Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: interaction between D1- and D2-like receptors J. Exp. Biol., July 15, 2006; 209(14): 2785 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dames, B. Zimmermann, R. Schmidt, J. Rein, M. Voss, B. Schewe, B. Walz, and O. Baumann cAMP regulates plasma membrane vacuolar-type H+-ATPase assembly and activity in blowfly salivary glands. PNAS, March 7, 2006; 103(10): 3926 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Serrano, G. Blanvillain, D. Soyez, G. Charmantier, E. Grousset, F. Aujoulat, and C. Spanings-Pierrot Putative involvement of crustacean hyperglycemic hormone isoforms in the neuroendocrine mediation of osmoregulation in the crayfish Astacus leptodactylus J. Exp. Biol., March 15, 2003; 206(6): 979 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tresguerres, H. Onken, A. F. Perez, and C. M. Luquet Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: rapid responses to osmotic variations J. Exp. Biol., February 1, 2003; 206(3): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Taylor and P. Greenaway Osmoregulation in the terrestrial Christmas Island red crab Gecarcoidea natalis (Brachyura: Gecarcinidae): modulation of branchial chloride uptake from the urine J. Exp. Biol., October 15, 2002; 205(20): 3251 - 3260. [Abstract] [Full Text] [PDF]
|
|
