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Journal of Experimental Biology, Vol 203, Issue 13 2025-2038, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
KC Daly and BH Smith
Department of Entomology, Ohio State University, Columbus 43210-1220, USA. daly.40@osu.edu
The proboscis extension response conditioning protocol has been used to explore olfactory-based associative learning in an array of insects. We have monitored a different feeding reflex, which involves activation of the cibarial pump, to demonstrate olfactory learning in the moth Manduca sexta. In the first experiment, four different treatment conditions were used to assess associative (Pavlovian) learning. The results indicate that an excitatory cibarial pump response develops and is retained for at least 24 h only when odor is forward-paired with the presentation of sucrose. Three control treatments, backward pairing, air (no odor) pairing and random pairing, failed to increase the cibarial pump response. However, an excitatory cibarial pump response developed when the backward- and air-paired groups received forward pairing of odor and sucrose on the following day. In contrast, moths experiencing random pairing on day 1 displayed a slower rate of acquisition during forward pairing on day 2, which may indicate inhibition. The second experiment investigated discrimination learning. Two odors were randomly presented, one odor being forward-paired with sucrose (+), the other presented alone (-) in a counterbalanced design. Again, only when odor was forward-paired with sucrose did learning occur. We discuss the implication of these findings for a broader comparative analysis of learning in insects.
This article has been cited by other articles:
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 E. K. Mwilaria, C. Ghatak, and K. C. Daly Disruption of GABAA in the Insect Antennal Lobe Generally Increases Odor Detection and Discrimination Thresholds Chem Senses, March 1, 2008; 33(3): 267 - 281. [Abstract] [Full Text] [PDF]
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K. C. Daly, L. A. Carrell, and E. Mwilaria Characterizing Psychophysical Measures of Discrimination Thresholds and the Effects of Concentration on Discrimination Learning in the Moth Manduca sexta Chem Senses, January 1, 2008; 33(1): 95 - 106. [Abstract] [Full Text] [PDF]
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 J. Goyret and R. A. Raguso The role of mechanosensory input in flower handling efficiency and learning by Manduca sexta J. Exp. Biol., May 1, 2006; 209(9): 1585 - 1593. [Abstract] [Full Text] [PDF]
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R. A. Raguso, A. R. LeClere, and B. O. Schlumpberger Sensory Flexibility in Hawkmoth Foraging Behavior: Lessons from Manduca sexta and Other Species Chem Senses, January 1, 2005; 30(suppl_1): i295 - i296. [Full Text] [PDF]
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K. C. Daly, T. A. Christensen, H. Lei, B. H. Smith, and J. G. Hildebrand Learning modulates the ensemble representations for odors in primary olfactory networks PNAS, July 13, 2004; 101(28): 10476 - 10481. [Abstract] [Full Text] [PDF]
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 K. C. Daly, G. A. Wright, and B. H. Smith Molecular Features of Odorants Systematically Influence Slow Temporal Responses Across Clusters of Coordinated Antennal Lobe Units in the Moth Manduca sexta J Neurophysiol, July 1, 2004; 92(1): 236 - 254. [Abstract] [Full Text] [PDF]
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 A. Balkenius, A. Kelber, and C. Balkenius A Model of Selection between Stimulus and Place Strategy in a Hawkmoth Adaptive Behavior, March 1, 2004; 12(1): 21 - 35. [Abstract] [PDF]
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G. A. Wright and B. H. Smith Different Thresholds for Detection and Discrimination of Odors in the Honey bee (Apis mellifera) Chem Senses, February 1, 2004; 29(2): 127 - 135. [Abstract] [Full Text] [PDF]
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 A. Kelber, A. Balkenius, and E. J. Warrant Colour Vision in Diurnal and Nocturnal Hawkmoths Integr. Comp. Biol., August 1, 2003; 43(4): 571 - 579. [Abstract] [Full Text] [PDF]
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K. C. Daly, S. Chandra, M. L. Durtschi, and B. H. Smith The generalization of an olfactory-based conditioned response reveals unique but overlapping odour representations in the moth Manduca sexta J. Exp. Biol., January 9, 2001; 204(17): 3085 - 3095. [Abstract] [Full Text] [PDF]
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