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Journal of Experimental Biology, Vol 202, Issue 4 417-428, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

A comparison of the release of a vasoactive-intestinal-peptide-like peptide and acetylcholine in the giant axon-Schwann cell preparation of the tropical squid Sepioteuthis sepioidea

PD Evans, V Reale, RM Merzon and J Villegas
The Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK and Centro de Neurociencias, Instituto International de Estudios Avanzados (IDEA), Apartado 17606, Parque Central, Caracas 1015-A, Venezuela. pde10@mole.bio.cam.ac.uk

A vasoactive intestinal peptide (VIP)-like peptide is released by axonal stimulation in the giant axon-Schwann cell preparation from the tropical squid Sepioteuthis sepioidea. It is also released by direct application of l-glutamate, the giant axon-Schwann cell signalling molecule in this preparation. The release of the peptide parallels the release of acetylcholine from the Schwann cells themselves in this preparation in a number of different ways. The release of both acetylcholine and the VIP-like peptide have the same threshold (between 2x10(-10) and 5x10(-10 )mol l-1) for l-glutamate application and the same recovery time after inhibition of release by exposure of the preparation to a prolonged pulse of l-glutamate. A prolonged l-glutamate pulse of 10(-8 )mol l-1 releases both substances for as long as the pulse is applied to the preparation, whereas a prolonged pulse of 10(-9 )mol l-1 l-glutamate releases acetylcholine in the same way but releases the VIP-like peptide only transiently. The VIP-like peptide is likely to be co-released with acetylcholine from the Schwann cells.
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© The Company of Biologists Ltd 1999