QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baker, J. D. Articles by Truman, J. W. Search for Related Content PubMed PubMed Citation Articles by Baker, J. D. Articles by Truman, J. W. Social Bookmarking                         What's this?

Journal of Experimental Biology, Vol 202, Issue 21 3037-3048, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

The hormonal coordination of behavior and physiology at adult ecdysis in Drosophila melanogaster

JD Baker, SL McNabb and JW Truman
Department of Zoology, University of Washington, Box 351800, Seattle, WA 98195, USA. jabaker@ms.cc.sunysb.edu

In insects, ecdysis is thought to be controlled by the interaction between peptide hormones; in particular between ecdysis-triggering hormone (ETH) from the periphery and eclosion hormone (EH) and crustacean cardioactive peptide (CCAP) from the central nervous system. We examined the behavioral and physiological functions of the first two of these peptides in Drosophila melanogaster using wild-type flies and knockout flies that lacked EH neurons. We used ETH from Manduca sexta (MasETH) to induce premature ecdysis and compared the responses of the two types of flies. The final release of EH normally occurs approximately 40 min before ecdysis. It is correlated with cyclic guanosine monophosphate (cGMP) production in selected neurons and tracheae, by an elevation in the heart rate and by the filling of the new tracheae with air. Injection of developing flies with MasETH causes all these events to occur prematurely. In EH cell knockouts, none of these changes occurs in response to MasETH, and these flies show a permanent failure in tracheal filling. This failure can be overcome in the knockouts by injecting them with membrane-permeant analogs of cGMP, the second messenger for EH. The basis for the 40 min delay between EH release and the onset of ecdysis was examined by decapitating flies at various times relative to EH release. In flies that had already released EH, decapitation was always followed within 1 min by the start of ecdysis. Immediate ecdysis was never observed when the EH cell knockout flies were decapitated. We propose that EH activates both ventral central nervous system elements necessary for ecdysis (possibly the CCAP cells) and descending inhibitory neurons from the head. This descending inhibition establishes a delay in the onset of ecdysis that allows the completion of EH-activated physiological processes such as tracheal filling. A waning in the inhibition eventually allows ecdysis to begin 30-40 min later.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				H. A. Woods, J. C. Sprague, and J. N. Smith Cavitation in the embryonic tracheal system of Manduca sexta J. Exp. Biol., October 15, 2009; 212(20): 3296 - 3304. [Abstract] [Full Text] [PDF]

				N. C. Peabody, J. B. Pohl, F. Diao, A. P. Vreede, D. J. Sandstrom, H. Wang, P. K. Zelensky, and B. H. White Characterization of the Decision Network for Wing Expansion in Drosophila Using Targeted Expression of the TRPM8 Channel J. Neurosci., March 18, 2009; 29(11): 3343 - 3353. [Abstract] [Full Text] [PDF]

				J. E. Natzle, J. A. Kiger Jr., and M. M. Green Bursicon Signaling Mutations Separate the Epithelial-Mesenchymal Transition From Programmed Cell Death During Drosophila melanogaster Wing Maturation Genetics, October 1, 2008; 180(2): 885 - 893. [Abstract] [Full Text] [PDF]

				P. Hari, M. Deshpande, N. Sharma, N. Rajadhyaksha, N. Ramkumar, K.-i. Kimura, V. Rodrigues, and S. Tole Chip Is Required for Posteclosion Behavior in Drosophila J. Neurosci., September 10, 2008; 28(37): 9145 - 9150. [Abstract] [Full Text] [PDF]

				S. L. McNabb and J. W. Truman Light and peptidergic eclosion hormone neurons stimulate a rapid eclosion response that masks circadian emergence in Drosophila J. Exp. Biol., July 15, 2008; 211(14): 2263 - 2274. [Abstract] [Full Text] [PDF]

				M. M. Davis, S. L. O'Keefe, D. A. Primrose, and R. B. Hodgetts A neuropeptide hormone cascade controls the precise onset of post-eclosion cuticular tanning in Drosophila melanogaster Development, December 15, 2007; 134(24): 4395 - 4404. [Abstract] [Full Text] [PDF]

				Y.-J. Kim, D. Zitnan, K.-H. Cho, D. A. Schooley, A. Mizoguchi, and M. E. Adams Central peptidergic ensembles associated with organization of an innate behavior PNAS, September 19, 2006; 103(38): 14211 - 14216. [Abstract] [Full Text] [PDF]

				K. K. Langlais, J. A. Stewart, and D. B. Morton Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system of Drosophila melanogaster J. Exp. Biol., June 1, 2004; 207(13): 2323 - 2338. [Abstract] [Full Text] [PDF]

				A. C. Clark, M. L. del Campo, and J. Ewer Neuroendocrine Control of Larval Ecdysis Behavior in Drosophila: Complex Regulation by Partially Redundant Neuropeptides J. Neurosci., April 28, 2004; 24(17): 4283 - 4292. [Abstract] [Full Text] [PDF]

				K.-i. Kimura, A. Kodama, Y. Hayasaka, and T. Ohta Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster Development, April 1, 2004; 131(7): 1597 - 1606. [Abstract] [Full Text] [PDF]

				J. H. Park, A. J. Schroeder, C. Helfrich-Forster, F. R. Jackson, and J. Ewer Targeted ablation of CCAP neuropeptide-containing neurons of Drosophila causes specific defects in execution and circadian timing of ecdysis behavior Development, June 15, 2003; 130(12): 2645 - 2656. [Abstract] [Full Text] [PDF]

				D. Zitnan, I. Zitnanova, I. Spalovska, P. Takac, Y. Park, and M. E. Adams Conservation of ecdysis-triggering hormone signalling in insects J. Exp. Biol., April 15, 2003; 206(8): 1275 - 1289. [Abstract] [Full Text] [PDF]

				I. E. Clark, K. C. Dobi, H. K. Duchow, A. N. Vlasak, and E. R. Gavis A common translational control mechanism functions in axial patterning and neuroendocrine signaling in Drosophila Development, March 9, 2003; 129(14): 3325 - 3334. [Abstract] [Full Text] [PDF]

				Y. Park, V. Filippov, S. S. Gill, and M. E. Adams Deletion of the ecdysis-triggering hormone gene leads to lethal ecdysis deficiency Development, March 3, 2003; 129(2): 493 - 503. [Abstract] [Full Text] [PDF]

				D. Zitnan, L. Hollar, I. Spalovska, P. Takac, I. Zitnanova, S. S. Gill, and M. E. Adams Molecular cloning and function of ecdysis-triggering hormones in the silkworm Bombyx mori J. Exp. Biol., November 15, 2002; 205(22): 3459 - 3473. [Abstract] [Full Text] [PDF]

				J. D. Baker and J. W. Truman Mutations in the Drosophila glycoprotein hormone receptor, rickets, eliminate neuropeptide-induced tanning and selectively block a stereotyped behavioral program J. Exp. Biol., September 1, 2002; 205(17): 2555 - 2565. [Abstract] [Full Text] [PDF]

				M. Fuse and J. W. Truman Modulation of ecdysis in the moth Manduca sexta: the roles of the suboesophageal and thoracic ganglia J. Exp. Biol., April 15, 2002; 205(8): 1047 - 1058. [Abstract] [Full Text] [PDF]

				I. Zitnanova, M. E. Adams, and D. Zitnan Dual ecdysteroid action on the epitracheal glands and central nervous system preceding ecdysis of Manduca sexta J. Exp. Biol., March 12, 2002; 204(20): 3483 - 3495. [Abstract] [Full Text] [PDF]

				G. F. Rast Nitric oxide induces centrally generated motor patterns in the locust suboesophageal ganglion J. Exp. Biol., January 11, 2001; 204(21): 3789 - 3801. [Abstract] [Full Text] [PDF]