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Journal of Experimental Biology, Vol 202, Issue 15 2059-2069, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
CJ Montpetit and SF Perry
Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5.
The goal of the present investigation was to assess the relative involvement of nicotinic and muscarinic cholinergic receptors in the neuronal control of catecholamine secretion from the chromaffin tissue of rainbow trout (Oncorhynchus mykiss). This was accomplished by first developing and validating a nerve-stimulating technique able specifically to activate the nerve fibres innervating the chromaffin cells in order to elicit secretion of catecholamines. Using an in situ saline-perfused posterior cardinal vein preparation, it was demonstrated that whole-body field stimulation caused specific voltage-dependent neuronal stimulation of adrenaline and noradrenaline secretion. The contribution of non-specific depolarization was negligible. Several experimental results confirmed the specificity of the field stimulation technique. First, pre-treatment with neostigmine (an anticholinesterase) prolonged and more than doubled the amount of adrenaline secreted in response to electrical stimulation. Second, pre-treatment with the nicotinic receptor antagonist hexamethonium inhibited the electrically evoked secretion of adrenaline and noradrenaline. Third, perfusion with Na+-free saline or removal of the spinal cord abolished secretion of both catecholamines in response to the electrical stimulus. By using the field stimulation technique, this study is the first to demonstrate conclusively a role for muscarinic receptors in catecholamine secretion from trout chromaffin cells. Specifically, muscarinic cholinergic stimulation enhances nicotinic-evoked secretion of catecholamines and, under intense stimulation, may directly cause secretion. The results of the present study suggest the presence of muscarinic receptors on rainbow trout chromaffin cells with a functional role in the cholinergic control of catecholamine secretion.
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B. McNeill and S. F. Perry Nitric oxide and the control of catecholamine secretion in rainbow trout Oncorhynchus mykiss J. Exp. Biol., June 15, 2005; 208(12): 2421 - 2431. [Abstract] [Full Text] [PDF]
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B. McNeill, C. J. Montpetit, and S. F. Perry Catecholamine secretion in trout chromaffin cells experiencing nicotinic receptor desensitization is maintained by non-cholinergic neurotransmission J. Exp. Biol., December 1, 2003; 206(23): 4247 - 4253. [Abstract] [Full Text] [PDF]
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C. J. Montpetit, A. Shahsavarani, and S. F. Perry Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss) J. Exp. Biol., June 1, 2003; 206(11): 1917 - 1927. [Abstract] [Full Text] [PDF]
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K. N. Lapner and S. F. Perry The role of angiotensin II in regulating catecholamine secretion during hypoxia in rainbow trout Oncorhynchus mykiss J. Exp. Biol., January 12, 2001; 204(23): 4169 - 4176. [Abstract] [Full Text] [PDF]
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S. Perry, C. Montpetit, and M Borowska The effects of acute hypoxia on chemically or neuronally induced catecholamine secretion in rainbow trout (Oncorhynchus mykiss) in situ and in vivo J. Exp. Biol., January 5, 2000; 203(9): 1487 - 1495. [Abstract] [PDF]
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K. Lapner, C. Montpetit, and S. Perry Desensitisation of chromaffin cell nicotinic receptors does not impede catecholamine secretion during acute hypoxia in rainbow trout (Oncorhynchus mykiss) J. Exp. Biol., January 5, 2000; 203(10): 1589 - 1597. [Abstract] [PDF]
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