|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 201, Issue 8 1163-1175, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
PA Burke and RO Poyton
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Eukaryotic cytochrome c oxidases are complex oligomeric membrane proteins composed of subunit polypeptides encoded by both nuclear and mitochondrial genomes. While the mitochondrially encoded subunits are encoded by unique genes, some of the nuclear-encoded subunits are encoded by multigene families. The isoforms produced by these multigene families are tissue-specific and/or developmentally regulated in mammals and environmentally regulated in lower eukaryotes. Isoforms for one of the subunits, V, in the yeast Saccharomyces cerevisiae and one of the subunits, VII, in the slime mold Dictyostelium discoideum are regulated differentially by oxygen concentration. Extensive studies with the yeast subunit V isoforms have revealed that the genes for these proteins are switched on or off at very low oxygen concentrations (0.5-1 micromol l-1 O2) and that they affect the catalytic properties of holocytochrome c oxidase differentially. By altering an internal step in electron transfer between heme a and the binuclear reaction center (composed of heme a3 and CuB), the 'hypoxic' isoform, Vb, enhances the catalytic constant three- to fourfold relative to the 'aerobic' isoform, Va. Modeling studies suggest that this occurs by an interaction between transmembrane helix VII of subunit I and the transmembrane helix in subunit V. The inverse regulation of these two isoforms allows cells to assemble different types of holoenzyme isoenzymes in response to oxygen concentration. Oxygen also regulates the level of transcription of the genes for the other nuclear-coded subunits of yeast cytochrome c oxidase and affects the level of two of the mitochondrially encoded subunits (I and II) post-transcriptionally. Thus, the level of cytochrome c oxidase activity that is produced at different oxygen tensions in yeast is determined in part by the number of holoenzyme molecules that are assembled and in part by the oxygen-regulated isoforms of subunit V. The possibility that this type of control exists in other organisms is considered.
This article has been cited by other articles:
|
|
 |
|
  P. R. Castello, D. K. Woo, K. Ball, J. Wojcik, L. Liu, and R. O. Poyton Reactive Oxygen Species Special Feature: Oxygen-regulated isoforms of cytochrome c oxidase have differential effects on its nitric oxide production and on hypoxic signaling PNAS, June 17, 2008; 105(24): 8203 - 8208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Spear, S. K. Prabu, D. Galati, H. Raza, H. K. Anandatheerthavarada, and N. G. Avadhani beta1-Adrenoreceptor activation contributes to ischemia-reperfusion damage as well as playing a role in ischemic preconditioning Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2459 - H2466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Fontanesi, I. C. Soto, D. Horn, and A. Barrientos Assembly of mitochondrial cytochrome c-oxidase, a complicated and highly regulated cellular process Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1129 - C1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Prabu, H. K. Anandatheerthavarada, H. Raza, S. Srinivasan, J. F. Spear, and N. G. Avadhani Protein Kinase A-mediated Phosphorylation Modulates Cytochrome c Oxidase Function and Augments Hypoxia and Myocardial Ischemia-related Injury J. Biol. Chem., January 27, 2006; 281(4): 2061 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cassanova, K. M. O'Brien, B. T. Stahl, T. McClure, and R. O. Poyton Yeast Flavohemoglobin, a Nitric Oxide Oxidoreductase, Is Located in Both the Cytosol and the Mitochondrial Matrix: EFFECTS OF RESPIRATION, ANOXIA, AND THE MITOCHONDRIAL GENOME ON ITS INTRACELLULAR LEVEL AND DISTRIBUTION J. Biol. Chem., March 4, 2005; 280(9): 7645 - 7653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Church, B. Goehring, D. Forsha, P. Wazny, and R. O. Poyton A Role for Pet100p in the Assembly of Yeast Cytochrome c Oxidase: INTERACTION WITH A SUBASSEMBLY THAT ACCUMULATES IN A pet100 MUTANT J. Biol. Chem., January 21, 2005; 280(3): 1854 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T Leussink, H. J Baelde, T. M B.-v. den Berg, E. de Heer, G. B van der Voet, A. Slikkerveer, J. A Bruijn, and F. A de Wolff Renal epithelial gene expression profile and bismuth-induced resistance against cisplatin nephrotoxicity Human and Experimental Toxicology, October 1, 2003; 22(10): 535 - 540. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Miyazaki, L. Neff, S. Tanaka, W. C. Horne, and R. Baron Regulation of cytochrome c oxidase activity by c-Src in osteoclasts J. Cell Biol., March 3, 2003; 160(5): 709 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dirmeier, K. M. O'Brien, M. Engle, A. Dodd, E. Spears, and R. O. Poyton Exposure of Yeast Cells to Anoxia Induces Transient Oxidative Stress. IMPLICATIONS FOR THE INDUCTION OF HYPOXIC GENES J. Biol. Chem., September 13, 2002; 277(38): 34773 - 34784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Radford, B. Wan, A. Richman, L. S. Szczepaniak, J.-L. Li, K. Li, K. Pfeiffer, H. Schagger, D. J. Garry, and R. W. Moreadith Cardiac dysfunction in mice lacking cytochrome-c oxidase subunit VIaH Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H726 - H733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakagawa, S. Sugioka, Y. Kaneko, and S. Harashima O2R, a Novel Regulatory Element Mediating Rox1p-Independent O2 and Unsaturated Fatty Acid Repression of OLE1 in Saccharomyces cerevisiae J. Bacteriol., January 15, 2001; 183(2): 745 - 751. [Abstract] [Full Text]
|
|
|
|
|
|
K. E. Kwast, P. V. Burke, B. T. Staahl, and R. O. Poyton Oxygen sensing in yeast: Evidence for the involvement of the respiratory chain in regulating the transcription of a subset of hypoxic genes PNAS, May 11, 1999; 96(10): 5446 - 5451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dagsgaard, L. E. Taylor, K. M. O'Brien, and R. O. Poyton Effects of Anoxia and the Mitochondrion on Expression of Aerobic Nuclear COX Genes in Yeast. EVIDENCE FOR A SIGNALING PATHWAY FROM THE MITOCHONDRIAL GENOME TO THE NUCLEUS J. Biol. Chem., March 2, 2001; 276(10): 7593 - 7601. [Abstract] [Full Text] [PDF]
|
|
