QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kloppenburg, P. Articles by HORner, M. Search for Related Content PubMed PubMed Citation Articles by Kloppenburg, P. Articles by HORner, M. Social Bookmarking                         What's this?

Journal of Experimental Biology, Vol 201, Issue 17 2529-2541, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Voltage-activated currents in identified giant interneurons isolated from adult crickets gryllus bimaculatus

P Kloppenburg and M HORner
Section of Neurobiology and Behavior, Cornell University, Seeley G. Mudd Hall, Ithaca, NY 14853, USA and Institute for Zoology and Anthropology, Department of Cell Biology, University of Gottingen, Berliner Strasse 28, D-37073 Gottingen, Germa.

The electrophysiological properties of cultured giant interneurons isolated from the terminal ganglion of adult crickets (Gryllus bimaculatus) were investigated using whole-cell patch-clamp techniques. To allow for unequivocal identification of these interneurons in cell culture, a protocol for fast and selective labeling of their cell bodies was established. Prior to cell dissociation, the giant interneurons were backfilled through their axons in situ with a fluorescent dye (dextran tetramethylrhodamine). In primary cell cultures, the cell bodies of giant interneurons were identified among a population of co-cultured neurons by their red fluorescence. Action potentials were recorded from the cell bodies of the cultured interneurons suggesting that several types of voltage-activated ion channels exist in these cells. Using voltage-clamp recording techniques, four voltage-activated currents were isolated and characterized. The giant interneurons express at least two distinct K+ currents: a transient current that is blocked by 4-aminopyridine (4x10(-3 )mol l-1) and a sustained current that is partially blocked by tetraethylammonium (3x10(-2 )mol l-1) and quinidine (2x10(-4 )mol l-1). In addition, a transient Na+ current sensitive to 10(-7 )mol l-1 tetrodotoxin and a Ca2+ current blocked by 5x10(-4 )mol l-1 CdCl2 have been characterized. This study represents the first step in an attempt to analyze the cellular and ionic mechanisms underlying plasticity in the well-characterized and behaviorally important giant interneuron pathway in insects.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. Husch, M. Paehler, D. Fusca, L. Paeger, and P. Kloppenburg Distinct Electrophysiological Properties in Subtypes of Nonspiking Olfactory Local Interneurons Correlate With Their Cell Type-Specific Ca2+ Current Profiles J Neurophysiol, November 1, 2009; 102(5): 2834 - 2845. [Abstract] [Full Text] [PDF]

				H. Demmer and P. Kloppenburg Intrinsic Membrane Properties and Inhibitory Synaptic Input of Kenyon Cells as Mechanisms for Sparse Coding? J Neurophysiol, September 1, 2009; 102(3): 1538 - 1550. [Abstract] [Full Text] [PDF]

				A. Husch, M. Paehler, D. Fusca, L. Paeger, and P. Kloppenburg Calcium Current Diversity in Physiologically Different Local Interneuron Types of the Antennal Lobe J. Neurosci., January 21, 2009; 29(3): 716 - 726. [Abstract] [Full Text] [PDF]

				H. Ogawa, G. I. Cummins, G. A. Jacobs, and K. Oka Dendritic Design Implements Algorithm for Synaptic Extraction of Sensory Information J. Neurosci., April 30, 2008; 28(18): 4592 - 4603. [Abstract] [Full Text] [PDF]

				A. Husch, S. Hess, and P. Kloppenburg Functional Parameters of Voltage-Activated Ca2+ Currents From Olfactory Interneurons in the Antennal Lobe of Periplaneta americana J Neurophysiol, January 1, 2008; 99(1): 320 - 332. [Abstract] [Full Text] [PDF]

				A. R. Mercer, P. Kloppenburg, and J. G. Hildebrand Plateau Potentials in Developing Antennal-Lobe Neurons of the Moth, Manduca sexta J Neurophysiol, April 1, 2005; 93(4): 1949 - 1958. [Abstract] [Full Text] [PDF]

				Z. Liu, W. Song, and K. Dong Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel PNAS, August 10, 2004; 101(32): 11862 - 11867. [Abstract] [Full Text] [PDF]

				B. Grunewald Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway J. Exp. Biol., January 1, 2003; 206(1): 117 - 129. [Abstract] [Full Text] [PDF]

				D. Wicher Peptidergic Modulation of an Insect Na+ Current: Role of Protein Kinase A and Protein Kinase C J Neurophysiol, January 1, 2001; 85(1): 374 - 383. [Abstract] [Full Text] [PDF]

				R. Hewes Voltage-dependent ionic currents in the ventromedial eclosion hormone neurons of Manduca sexta J. Exp. Biol., January 9, 1999; 202(17): 2371 - 2383. [Abstract] [PDF]

				C Benkenstein, M Schmidt, and M Gewecke Voltage-activated whole-cell K+ currents in lamina cells of the desert locust schistocerca gregaria J. Exp. Biol., January 7, 1999; 202(14): 1939 - 1951. [Abstract] [PDF]