spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Buckingham, S.
Right arrow Articles by Sattelle, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buckingham, S.
Right arrow Articles by Sattelle, F.

Journal of Experimental Biology, Vol 200, Issue 21 2685-2692, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Imidacloprid actions on insect neuronal acetylcholine receptors

S Buckingham, B Lapied, H Corronc and F Sattelle

The neonicotinoid insecticide Imidacloprid acts at three pharmacologically distinct acetylcholine receptor (AChR) subtypes in the cockroach (Periplaneta americana) nervous system, but is ineffective on muscarinic receptors. Imidacloprid (3-100µmoll-1) induced dose-dependent depolarizations at cockroach cercal afferent/giant interneurone synapses. These responses were insensitive to 20µmoll-1 atropine but were completely blocked by the nicotinic antagonist mecamylamine (50µmoll-1). Similarly, Imidacloprid-induced depolarizations of cultured cockroach dorsal unpaired median (DUM) neurones dissociated from the same (terminal abdominal) ganglion were also completely blocked by 100µmoll-1 mecamylamine. However, two components of the response could be distinguished on the basis of their differential sensitivities to 0.1µmoll-1-bungarotoxin (-BTX), which selectively blocks AChRs with 'mixed' nicotinic/muscarinic pharmacology in this preparation. This indicates that Imidacloprid affects both AChRs sensitive to -BTX and -BTX-insensitive nicotinic acetylcholine receptors (nAChRs). Thus, in the cockroach, Imidacloprid activates -BTX-sensitive synaptic nAChRs in giant interneurones, -BTX-insensitive extrasynaptic nAChRs in DUM neurones, and a recently characterized DUM neurone 'mixed' AChR that is sensitive to both nicotinic and muscarinic ligands. Imidacloprid does not act on muscarinic acetylcholine receptors (mAChRs) present on DUM neurone cell bodies and at the cercal afferent/giant interneurone synapses. This study shows that Imidacloprid can act on pharmacologically diverse nAChR subtypes.


This article has been cited by other articles:


Home page
 J. Environ. Qual.Home page
C. W. Ingram, M. S. Coyne, and D. W. Williams
Effects of Commercial Diazinon and Imidacloprid on Microbial Urease Activity in Soil and Sod
J. Environ. Qual., August 9, 2005; 34(5): 1573 - 1580.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Dugravot, F. Grolleau, D. Macherel, A. Rochetaing, B. Hue, M. Stankiewicz, J. Huignard, and B. Lapied
Dimethyl Disulfide Exerts Insecticidal Neurotoxicity Through Mitochondrial Dysfunction and Activation of Insect KATP Channels
J Neurophysiol, July 1, 2003; 90(1): 259 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. Courjaret and B. Lapied
Complex Intracellular Messenger Pathways Regulate One Type of Neuronal alpha -Bungarotoxin-Resistant Nicotinic Acetylcholine Receptors Expressed in Insect Neurosecretory Cells (Dorsal Unpaired Median Neurons)
Mol. Pharmacol., July 1, 2001; 60(1): 80 - 91.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1997