spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chao, H.
Right arrow Articles by Carpenter, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chao, H.
Right arrow Articles by Carpenter, J. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Experimental Biology, Vol 199, Issue 9 2071-2076, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

Effects of antifreeze proteins on red blood cell survival during cryopreservation

H Chao, PL Davies and JF Carpenter
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.

Antifreeze protein (AFP) types, I, II and III were tested for their ability to protect red blood cells from lysis during warming, after cryopreservation in hydroxyethyl starch. All three types reduced hemolysis to 25% of control values at similar micromolar concentrations but enhanced lysis as the AFP concentration approached millimolar levels. Site-directed mutants of type III AFP with different thermal hysteresis activities were tested for their ability to protect the cryopreserved cells from lysis. Their relative efficacy in protecting the cells correlated closely with their thermal hysteresis activity. Cryomicroscopy indicated that the protection of red cells by type III AFP and the mutant forms was due to inhibition of ice recrystallization.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Nishimiya, S. Ohgiya, and S. Tsuda
Artificial Multimers of the Type III Antifreeze Protein: EFFECTS ON THERMAL HYSTERESIS AND ICE CRYSTAL MORPHOLOGY
J. Biol. Chem., August 22, 2003; 278(34): 32307 - 32312.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Fairley, B. J. Westman, L. H. Pham, A. D. J. Haymet, M. M. Harding, and J. P. Mackay
Type I Shorthorn Sculpin Antifreeze Protein. RECOMBINANT SYNTHESIS, SOLUTION CONFORMATION, AND ICE GROWTH INHIBITION STUDIES
J. Biol. Chem., June 28, 2002; 277(27): 24073 - 24080.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1996