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Journal of Experimental Biology, Vol 199, Issue 7 1579-1585, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
S Webster
The effects of emersion stress upon circulating hyperglycaemic hormone (CHH) levels in the edible crab Cancer pagurus were investigated using a highly specific and sensitive radioimmunoassay, with an antiserum directed against HPLC-purified C. pagurus CHH. Emersion resulted in hyperglycaemia and immediate hypoxia, as shown by rapid hyperlactaemia. CHH levels increased dramatically during the first hour of emersion, from almost undetectable levels to around 17 pmol l-1, thereafter increasing to around 30 pmol l-1 after 4 h of emersion. Short-term air exposure experiments demonstrated that significant increases in CHH levels (up to 3.5 pmol l-1) could be detected during the first 15 min of emersion. Although CHH appears to be fairly stable in haemolymph in vitro, injected CHH was cleared extremely rapidly from the haemolymph in vivo. The results suggest that emersion results in rapid, massive and prolonged exocytosis of CHH from the sinus gland. The sensitivity of the assay and the utility of this crab model may be useful in further studies to elucidate the control of CHH release in crustaceans.
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  Y.-W. A. Hsu, D. I. Messinger, J. S. Chung, S. G. Webster, H. O. de la Iglesia, and A. E. Christie Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs of Cancer crabs: implications for differential release and pleiotropic function J. Exp. Biol., August 15, 2006; 209(16): 3241 - 3256. [Abstract] [Full Text] [PDF]
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S. L. Tamone, M. M. Adams, and J. M. Dutton Effect of Eyestalk-Ablation on Circulating Ecdysteroids in Hemolymph of Snow Crabs, Chionoecetes opilio: Physiological Evidence for a Terminal Molt Integr. Comp. Biol., January 1, 2005; 45(1): 166 - 171. [Abstract] [Full Text] [PDF]
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S. Lorenzon, P. Edomi, P. G. Giulianini, R. Mettulio, and E. A. Ferrero Variation of crustacean hyperglycemic hormone (cHH) level in the eyestalk and haemolymph of the shrimp Palaemon elegans following stress J. Exp. Biol., November 15, 2004; 207(24): 4205 - 4213. [Abstract] [Full Text] [PDF]
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E. S. Chang Crustacean Hyperglycemic Hormone Family: Old Paradigms and New Perspectives Integr. Comp. Biol., June 1, 2001; 41(3): 380 - 388. [Abstract] [Full Text] [PDF]
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D. W. Borst, J. Ogan, B. Tsukimura, T. Claerhout, and K. C. Holford Regulation of the Crustacean Mandibular Organ Integr. Comp. Biol., June 1, 2001; 41(3): 430 - 441. [Abstract] [Full Text] [PDF]
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M. Phlippen, S. Webster, J. Chung, and H Dircksen Ecdysis of decapod crustaceans is associated with a dramatic release of crustacean cardioactive peptide into the haemolymph J. Exp. Biol., January 2, 2000; 203(3): 521 - 536. [Abstract] [PDF]
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 J. S. Chung, H. Dircksen, and S. G. Webster A remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas PNAS, November 9, 1999; 96(23): 13103 - 13107. [Abstract] [Full Text] [PDF]
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