QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schwarzbaum, P. Articles by Wieser, C. Search for Related Content PubMed PubMed Citation Articles by Schwarzbaum, P. Articles by Wieser, C. Social Bookmarking                         What's this?

Journal of Experimental Biology, Vol 199, Issue 7 1515-1521, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

Effect of chemical anoxia on protein kinase C and Na+, K+-ATPase in hepatocytes of goldfish (Carassius auratus) and rainbow trout (Oncorhynchus mykiss)

P Schwarzbaum, R Bernabeu, G Krumschnabel and C Wieser

Protein kinase C (PKC) and Na+/K+-ATPase in hepatocytes from the anoxia-tolerant goldfish (Carassius auratus) and the anoxia-intolerant rainbow trout (Oncorhynchus mykiss) were studied to determine their role in the anoxic response of these cells. PKC and Na+/K+-ATPase activities were measured for up to 90 min in the absence (normoxia) and presence (chemical anoxia) of 2 mmol l-1 sodium cyanide. PKC activity of normoxic cells from both species remained constant for the entire experimental period. Addition of cyanide had no effect on PKC activity of trout cells, which was maintained at 25 % of maximal PKC activity. In goldfish hepatocytes, PKC activity remained constant at 56 % of maximal PKC activity for 30 min but fell to 27 % after 90 min of anoxic exposure. ATPase activity was measured in hepatocytes exposed to 100 nmol l-1 phorbol-12,13-dibutyrate (PdBu), a treatment which enhanced PKC activity to its maximum level. In trout cells, there was no significant change in Na+/K+-ATPase activity whereas in goldfish hepatocytes a significant increase to about 150 % of the respective controls was observed. On the basis of the experimental evidence that in hepatocytes of goldfish (1) PKC and Na+/K+-ATPase activities decreased in parallel during chemical anoxia and (2) a stimulation of PKC activity by PdBu increased Na+/K+-ATPase activity, we postulate that PKC activity in goldfish, but not in trout, may be implicated in the Na+/K+-ATPase inhibition observed under anoxia.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				G. Krumschnabel, R. Gstir, C. Manzl, C. Prem, D. Pafundo, and P. J. Schwarzbaum Metabolic and ionic responses of trout hepatocytes to anisosmotic exposure J. Exp. Biol., June 1, 2003; 206(11): 1799 - 1808. [Abstract] [Full Text] [PDF]

				G Krumschnabel, P. Schwarzbaum, J Lisch, C Biasi, and W Wieser Oxygen-dependent energetics of anoxia-tolerant and anoxia-intolerant hepatocytes J. Exp. Biol., January 3, 2000; 203(5): 951 - 959. [Abstract] [PDF]

				P. J. Schwarzbaum, M. E. Frischmann, G. Krumschnabel, R. C. Rossi, and W. Wieser Functional role of ecto-ATPase activity in goldfish hepatocytes Am J Physiol Regulatory Integrative Comp Physiol, April 1, 1998; 274(4): R1031 - R1038. [Abstract] [Full Text] [PDF]