spacer gif spacer gif spacer gif spacer gif Online submission spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heming, T. A.
Right arrow Articles by Bidani, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heming, T. A.
Right arrow Articles by Bidani, A.

Journal of Experimental Biology, Vol 198, Issue 8 1711-1715, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH-regulatory role of plasma membrane V-ATPases

TA Heming, DL Traber, F Hinder and A Bidani
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555-0876, USA.

The role of plasma membrane V-ATPase activity in the regulation of cytosolic pH (pHi) was determined for resident alveolar and peritoneal macrophages (m theta) from sheep. Cytosolic pH was measured using 2',7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF). The baseline pHi of both cell types was sensitive to the specific V-ATPase inhibitor bafilomycin A1. Bafilomycin A1 caused a significant (approximately 0.2 pH units) and rapid (within seconds) decline in baseline pHi. Further, bafilomycin A1 slowed the initial rate of pHi recovery (dpHi/dt) from intracellular acid loads. Amiloride had no effects on baseline pHi, but reduced dpHi/dt (acid-loaded pHi nadir < 6.8) by approximately 35%. Recovery of pHi was abolished by co-treatment of m theta with bafilomycin A1 and amiloride. These data indicate that plasma membrane V-ATPase activity is a major determinant of pHi regulation in resident alveolar and peritoneal m theta from sheep. Sheep m theta also appear to possess a Na+/H+ exchanger. However, Na+/H+ exchange either is inactive or can be effectively masked by V-ATPase-mediated H+ extrusion at physiological pHi values.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Yang, A. K. Gillingham, A. Hodel, F. Koumanov, B. Woodward, and G. D. Holman
Insulin-stimulated cytosol alkalinization facilitates optimal activation of glucose transport in cardiomyocytes
Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1299 - E1307.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. A. Skinner, L. A. MacLaren, and A. G. Wildeman
Stage-dependent Redistribution of the V-ATPase During Bovine Implantation
J. Histochem. Cytochem., October 1, 1999; 47(10): 1247 - 1254.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Jayadev, J. G. Petranka, S. K. Cheran, J. A. Biermann, J. C. Barrett, and E. Murphy
Reduced Capacitative Calcium Entry Correlates with Vesicle Accumulation and Apoptosis
J. Biol. Chem., March 19, 1999; 274(12): 8261 - 8268.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Bowman, F. J. O'Neill, and B. J. Bowman
Mutations of pma-1, the Gene Encoding the Plasma Membrane H+-ATPase of Neurospora crassa, Suppress Inhibition of Growth by Concanamycin A, a Specific Inhibitor of Vacuolar ATPases
J. Biol. Chem., June 6, 1997; 272(23): 14776 - 14786.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1995