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Journal of Experimental Biology, Vol 198, Issue 7 1603-1611, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Small-diameter white myotomal muscle fibres associated with growth hyperplasia in the carp (Cyprinus carpio) express a distinct myosin heavy chain gene

S Ennion, L Gauvry, P Butterworth and G Goldspink
Department of Anatomy and Developmental Biology, Royal Free Hospital School of Medicine, University of London, UK.

A carp myosin heavy chain gene isoform was isolated from a genomic clone, restriction mapped and partially sequenced to reveal the location of various exons. The clone contains a complete gene of approximately 12.0 kb which is half the size of the corresponding mammalian and avian myosin heavy chain genes. The mRNA transcript of this gene, however, is the same size as mammalian and avian striated muscle myosin heavy chain genes (about 6000 nucleotides), illustrating that the difference in size at the genomic level is due to shorter introns. A 169 bp NsiI restriction fragment containing only the 3' untranslated region of this gene was subcloned and used as an isoform-specific probe to study the expression of this particular isoform. Hybridisation analysis could only detect expression of this myosin heavy chain gene in the white muscle of adult carp that had been subjected to an increased environmental temperature. No expression of this gene was detected in carp under 1 year of age. In situ hybridisation demonstrated that expression of this gene is limited to small-diameter white muscle fibres of adult carp, which are thought to be responsible for muscle growth by fibre hyperplasia.


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S Ennion, D Wilkes, L Gauvry, H Alami-Durante, and G Goldspink
Identification and expression analysis of two developmentally regulated myosin heavy chain gene transcripts in carp (Cyprinus carpio)
J. Exp. Biol., January 5, 1999; 202(9): 1081 - 1090.
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H.-W. Sun, C.-F. Hui, and J.-L. Wu
Cloning, Characterization, and Expression in Escherichia coli of Three Creatine Kinase Muscle Isoenzyme cDNAs from Carp (Cyprinus carpio) Striated Muscle
J. Biol. Chem., December 11, 1998; 273(50): 33774 - 33780.
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© The Company of Biologists Ltd 1995