|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 196, Issue 1 347-360, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
WF Boron, SJ Waisbren, IM Modlin and JP Geibel
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut.
Although many factors can influence intracellular pH (pHi), some of the most important are those that involve the movement of acids and bases across the cell membrane. We will discuss recent results concerning barriers to the movement of H+, NH3 and CO2 across the apical cell membranes of gastric gland cells. Cell membranes are generally highly permeable to small, lipophilic molecules such as NH3 and CO2. In fact, only two examples are known of membranes relatively impermeable to NH3 and none membranes permeable to CO2. We recently developed a technique for perfusing the lumen of a single hand-dissected gastric gland on the stage of a microscope, while monitoring pHi with a fluorescent dye. We observed the expected pHi changes when we exposed the basolateral (i.e. blood-side) membrane to a pH 6.4 solution (a large, rapid pHi decrease), to a pH 7.4 solution containing approximately 0.3 mmoll-1 NH3 (a large and rapid pHi increase) or to a pH7.4 solution equilibrated with 1% CO2 (a rapid pHi decrease of -0.08). However, pHi was not significantly affected by perfusing the lumen with a pH 1.4 solution, with a pH 7.4 solution containing as much as 2.7 mmoll-1 NH3 or with a pH 6.1 solution equilibrated with 100% CO2. These data indicate that a barrier at or near the apical membrane has a uniquely low permeability to H+, NH3 and CO2.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K.-H. Han, K. Mekala, V. Babida, H.-Y. Kim, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung Am J Physiol Lung Cell Mol Physiol, July 1, 2009; 297(1): L153 - L163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Endeward and G. Gros Extra- and intracellular unstirred layer effects in measurements of CO2 diffusion across membranes - a novel approach applied to the mass spectrometric 18O technique for red blood cells J. Physiol., March 15, 2009; 587(6): 1153 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Wallace Prostaglandins, NSAIDs, and Gastric Mucosal Protection: Why Doesn't the Stomach Digest Itself? Physiol Rev, October 1, 2008; 88(4): 1547 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Worrell, L. Merk, and J. B. Matthews Ammonium transport in the colonic crypt cell line, T84: role for Rhesus glycoproteins and NKCC1 Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G429 - G440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Endeward and G. Gros Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon J. Physiol., August 15, 2005; 567(1): 253 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Messerli, L. A. Amaral-Zettler, E. Zettler, S.-K. Jung, P. J. S. Smith, and M. L. Sogin Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile J. Exp. Biol., July 1, 2005; 208(13): 2569 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Allen and G. Flemstrom Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin Am J Physiol Cell Physiol, January 1, 2005; 288(1): C1 - C19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Sutto, G. E Conner, and M. Salathe Regulation of human airway ciliary beat frequency by intracellular pH J. Physiol., October 15, 2004; 560(2): 519 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nakamura and S. J. Hagen Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1264 - G1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zeiske, H. Meyer, and H. Wieczorek Insect midgut K+ secretion: concerted run-down of apical/basolateral transporters with extra-/intracellular acidity J. Exp. Biol., February 15, 2002; 205(4): 463 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Hagen, H. Wu, and S. W. Morrison NH4Cl inhibition of acid secretion: possible involvement of an apical K+ channel in bullfrog oxyntic cells Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G400 - G410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Geers and G. Gros Carbon Dioxide Transport and Carbonic Anhydrase in Blood and Muscle Physiol Rev, April 1, 2000; 80(2): 681 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Akiba and J. D. Kaunitz Regulation of intracellular pH and blood flow in rat duodenal epithelium in vivo Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G293 - G302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Debellis, R. Caroppo, E. Fromter, and S. Curci Alkaline secretion by frog gastric glands measured with pH microelectrodes in the gland lumen J. Physiol., November 15, 1998; 513(1): 235 - 241. [Abstract] [Full Text] [PDF]
|
|
