|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 196, Issue 1 319-335, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
GA Ahearn, Z Zhuang, J Duerr and V Pennington
Department of Zoology, University of Hawaii at Manoa, Honolulu 96822.
In recent years, an electrogenic 2Na+/1H+ antiporter has been identified in a variety of invertebrate epithelial brush-border membranes of gut, kidney and gill tissues. The antiporter differs significantly in its physiological properties from the electroneutral 1Na+/1H+ antiporter proposed for vertebrate cells. In all invertebrate cells examined, the antiporter displayed a 2:1 transport stoichiometry, responded to an induced transmembrane potential and exhibited a high binding affinity for the divalent cation Ca2+, which acted as a competitive inhibitor of Na+ transport. A monoclonal antibody specific for the crustacean electrogenic antiporter inhibited 2Na+/1H+ exchange, but was without effect on Na(+)-dependent D-glucose transport. Immunoreactivity was localized at hepatopancreatic brush-border and vacuolar membranes, antennal gland coelomosac podocytes and posterior gill epithelial cells-all locations were published reports described unique cation exchange kinetics. Significant fractions of Ca2+ transport into invertebrate cells across brush-border membranes occurred by an electrogenic, amiloride-sensitive exchange process, probably by the 2Na+/1H+ antiporter, and this transport was markedly inhibited by exogenous zinc and cadmium. A recently identified electroneutral, amiloride-sensitive, hepatopancreatic epithelial basolateral Na+/H+ antiporter was uninfluenced by the brush-border monoclonal antibody, exhibited an apparent 1:1 transport stoichiometry and possessed a minimal divalent cation specificity. Calcium transport at this epithelial pole occurred by the combination of a Ca2+/Na+ antiporter, an ATP-dependent Ca(2+)-ATPase and a verapamil-sensitive calcium channel. These crustacean brush-border and basolateral transporters may play significant roles in calcification and heavy metal detoxification.
This article has been cited by other articles:
|
|
 |
|
  A. Bianchini and C. M. Wood Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss J. Exp. Biol., February 15, 2008; 211(4): 539 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Ebanks and M. Grosell Fluid and osmolyte recovery in the common pond snail Lymnaea stagnalis following full-body withdrawal J. Exp. Biol., February 1, 2008; 211(3): 327 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Conrad and G. A. Ahearn 3H-L-histidine and 65Zn2+ are cotransported by a dipeptide transport system in intestine of lobster Homarus americanus J. Exp. Biol., January 15, 2005; 208(2): 287 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Pullikuth, V. Filippov, and S. S. Gill Phylogeny and cloning of ion transporters in mosquitoes J. Exp. Biol., November 1, 2003; 206(21): 3857 - 3868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Chavez-Crooker, N. Garrido, and G. A. Ahearn Copper transport by lobster (Homarus americanus) hepatopancreatic mitochondria J. Exp. Biol., February 1, 2002; 205(3): 405 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Chavez-Crooker, N Garrido, and G. Ahearn Copper transport by lobster hepatopancreatic epithelial cells separated by centrifugal elutriation: measurements with the fluorescent dye Phen Green J. Exp. Biol., January 4, 2001; 204(8): 1433 - 1444. [Abstract] [PDF]
|
|
|
|
 |
|
 O. W. MOE Acute Regulation of Proximal Tubule Apical Membrane Na/H Exchanger NHE-3: Role of Phosphorylation, Protein Trafficking, and RegulatoryFactors J. Am. Soc. Nephrol., November 1, 1999; 10(11): 2412 - 2425. [Full Text]
|
|
|
|
|
|
M. Monteilh-Zoller, V Zonno, C Storelli, and G. Ahearn Effects of zinc on l- J. Exp. Biol., January 11, 1999; 202(21): 3003 - 3010. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Wunsch, C. P. Sanchez, M. Gekle, L. Grosse-Wortmann, J. Wiesner, and M. Lanzer Differential Stimulation of the Na+/H+ Exchanger Determines Chloroquine Uptake in Plasmodium falciparum J. Cell Biol., January 26, 1998; 140(2): 335 - 345. [Abstract] [Full Text] [PDF]
|
|
