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Journal of Experimental Biology, Vol 195, Issue 1 307-328, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
SJ Kleene, RC Gesteland and SH Bryant
Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati, OH 45267-0521.
Individual olfactory receptor neurons vary widely in their responses to odorants. Olfactory stimulus reception occurs in the cilia of the receptor neurons. Thus, the variability among individual neurons could in part be due to differences among the olfactory cilia. We have quantified the known conductance properties of each of 117 frog olfactory cilia. From a strictly qualitative viewpoint, the cilia were very homogeneous. All but a few of them had a basal conductance in the absence of odorants and second messengers, conductances stimulated by cytoplasmic cyclic AMP and by Ca2+ and a conductance measured in the presence of ATP and stimulated by GTP gamma S. However, the magnitudes of the conductances varied widely among the cilia. Amplitudes of the cyclic-AMP- and Ca(2+)-activated ciliary currents correlated strongly with one another across the 117 cilia and 24 frogs studied, suggesting that expression of the underlying channels may be co-regulated. None of the conductance properties correlated strongly with ciliary length, a marker of cell maturity. Given cytoplasmic MgATP as substrate, ciliary adenylate cyclase apparently produced cyclic AMP, which in turn gated membrane channels and increased the ciliary conductance. In some cilia, MgATP alone caused a very large increase in conductance. In others, there was little effect unless GTP gamma S, which increases cyclase activity, was also added. No effect of cytoplasmic inositol trisphosphate on ciliary conductance was detectable.
This article has been cited by other articles:
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  R. J. Flannery, D. A. French, and S. J. Kleene Clustering of Cyclic-Nucleotide-Gated Channels in Olfactory Cilia Biophys. J., July 1, 2006; 91(1): 179 - 188. [Abstract] [Full Text] [PDF]
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 W. Bonigk, J. Bradley, F. Muller, F. Sesti, I. Boekhoff, G. V. Ronnett, U. B. Kaupp, and S. Frings The Native Rat Olfactory Cyclic Nucleotide-Gated Channel Is Composed of Three Distinct Subunits J. Neurosci., July 1, 1999; 19(13): 5332 - 5347. [Abstract] [Full Text] [PDF]
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S. J. Kleene Both External and Internal Calcium Reduce the Sensitivity of the Olfactory Cyclic-Nucleotide-Gated Channel to CAMP J Neurophysiol, June 1, 1999; 81(6): 2675 - 2682. [Abstract] [Full Text] [PDF]
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D. Reuter, K. Zierold, W. H. Schroder, and S. Frings A Depolarizing Chloride Current Contributes to Chemoelectrical Transduction in Olfactory Sensory Neurons In Situ J. Neurosci., September 1, 1998; 18(17): 6623 - 6630. [Abstract] [Full Text] [PDF]
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T. Leinders-Zufall, C. A. Greer, G. M. Shepherd, and F. Zufall Imaging Odor-Induced Calcium Transients in Single Olfactory Cilia: Specificity of Activation and Role in Transduction J. Neurosci., August 1, 1998; 18(15): 5630 - 5639. [Abstract] [Full Text] [PDF]
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