spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uesaka, T.
Right arrow Articles by Ando, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uesaka, T.
Right arrow Articles by Ando, M.

Journal of Experimental Biology, Vol 188, Issue 1 205-216, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

Somatostatin-related peptides isolated from the eel gut: effects on ion and water absorption across the intestine of the seawater eel

T Uesaka, K Yano, M Yamasaki, K Nagashima and M Ando
Laboratory of Physiology, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.

Four somatostatin-related peptides were isolated from eel guts. Two of them were the same as eel SS-25II (eSS-25II) and eel SS-25I (eSS-25I) isolated from European eel pancreas. The remaining two peptides were C-terminal tetradecapeptides (eSS-14II and eSS-14I) of eSS-25II and eSS-25I, respectively. These four peptides all enhanced the serosa-negative transepithelial potential difference and short-circuit current across the seawater eel intestine after pretreatment with isobutylmethylxanthine, serotonin (5-HT) and methacholine, an agonist of acetylcholine (ACh). Among these peptides, eSS-25II was the most potent enhancer, followed by eSS-25I and eSS-14II. Since the large peptide (eSS-25II) acts at a lower concentration than the small somatostatin (eSS-14II), the 11 N-terminal amino acid residues seem to potentiate somatostatin action in the eel intestine. In contrast, eSS-14II was more potent than mammalian SS-14, indicating that the three amino acid residues (Tyr18, Gly21, Pro22) in the C-terminal portion also contribute to the potency of somatostatin. Endogenous somatostatin (eSS-25II) activated net Na+, Cl- and water fluxes across the seawater eel intestine. This stimulatory action was not inhibited by tetrodotoxin or yohimbine, an adrenergic antagonist, indicating that eSS-25II does not act through neuronal firing or through catecholamine release. Thus, eel somatostatins may act directly on the enterocytes, but on a distinct receptor from that for adrenaline, to antagonize the inhibition of NaCl and water absorption by 5-HT and ACh in the seawater eel intestine.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
W. S. Marshall, J. A. Howard, R. R. F. Cozzi, and E. M. Lynch
NaCl and fluid secretion by the intestine of the teleost Fundulus heteroclitus: involvement of CFTR
J. Exp. Biol., March 15, 2002; 205(6): 745 - 758.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
S. D. McCormick
Endocrine Control of Osmoregulation in Teleost Fish
Integr. Comp. Biol., August 1, 2001; 41(4): 781 - 794.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
T. Uesaka, K. Yano, S. Sugimoto, and M. Ando
Glucagon-like peptide isolated from the eel intestine: effects on atrial beating
J. Exp. Biol., January 9, 2001; 204(17): 3019 - 3026.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1994