spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ushio, H.
Right arrow Articles by Iino, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ushio, H.
Right arrow Articles by Iino, M.

Journal of Experimental Biology, Vol 181, Issue 1 95-105, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

CRAYFISH SKELETAL MUSCLE REQUIRES BOTH INFLUX OF EXTERNAL Ca2+ AND Ca2+ RELEASE FROM INTERNAL STORES FOR CONTRACTION

H. Ushio, S. Watabe and M. Iino

The isometric tension and membrane potential of single skeletal muscle fibres from the flexor muscle of the carpopodite in the meropodite of crayfish Procambarus clarkii (Girard) were studied to determine whether crayfish muscle contraction requires Ca2+ release from the sarcoplasmic reticulum. Contraction elicited by brief extracellular electrical stimulation was reduced by the removal of Ca2+ or by the addition of 25 micromolar nicardipine in crayfish Ringer's solution. Addition of 30 micromolar ryanodine with 1 mmol l-1 caffeine induced a transient contracture, the peak tension of which was 10-30 % of that of the high-K+ induced contracture and which declined to the pretreatment level in 20-60 min. After ryanodine-caffeine treatment, 30 mmol l-1 caffeine failed to induce contraction, suggesting that intracellular Ca2+ stores had been exhausted by the treatment. Extracellular electrical stimulation also failed to induce contraction after ryanodine-caffeine treatment, although the resting potential was not changed. These results suggest that Ca2+ release from the sarcoplasmic reticulum, together with Ca2+ influx via nicardipine-sensitive Ca2+ channels, is essential to the contraction of crayfish leg muscle fibres after a brief membrane depolarization.




© The Company of Biologists Ltd 1993