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Journal of Experimental Biology, Vol 181, Issue 1 279-293, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

METABOLIC CONTROL OF LUMINESCENCE IN ISOLATED PHOTOPHORES OF PORICHTHYS: EFFECTS OF GLUCOSE ON OXYGEN CONSUMPTION AND LUMINESCENCE

J. Mallefet and F. Baguet

1. Basal oxygen consumption of isolated photophores from Porichthys sp. at rest, i.e. without light emission, increased significantly from 0.101+/- 0.021 nmol min-1 to 0.173+/-0.016 nmol min-1 in response to the addition of 5.5 mmol l-1 glucose. 2. 5.5 mmol l-1 glucose pretreatment modified the time course of the two phases of adrenaline-induced luminescence; an increase in oxygen consumption was observed during the fast phase of light production but a decrease occurred during the slow phase of luminescence. 3. Pretreatment of isolated photophores with 5.5 mmol l-1 glucose totally inhibited the light emission induced by 1 mmol l-1 potassium cyanide. With this treatment, the respiration rate decreased progressively and after 40 min reached a value not significantly different from zero. 4. Even after blockage of cellular respiration by cyanide, an increase in the rate of oxygen consumption was observed during the fast adrenaline- induced luminescence. 5. Glucose utilisation by glycolysis or by oxidative metabolism may provide energy to an inhibitory mechanism that maintains the photophores in a non- luminescent state. 6. We suggest that the oxygen consumed during the fast phase of adrenaline luminescence could represent the activity of an extramitochondrial oxidative pathway involved in the light reaction.




© The Company of Biologists Ltd 1993