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Journal of Experimental Biology, Vol 181, Issue 1 27-48, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
J. E. Keen, D. M. Vianzon, A. P. Farrell and G. F. Tibbits
We examined the effect of temperature acclimation on the sensitivity of the rainbow trout heart to adrenaline and on the density of beta-adrenoceptors. The sensitivity of the heart was assessed using in situ working perfused heart and in vitro isometric ventricular strip preparations. When tested in situ and at acclimation temperature, hearts from fish acclimated to 8°C were approximately 10-fold more sensitive to adrenaline-supplemented perfusate than were hearts from fish acclimated to 18°C. The concentrations required for half-maximal stimulation (EC50) of myocardial power output were 1.9x10- 8 mol l-1 adrenaline and 1.7x10-7 mol l-1 adrenaline for hearts acclimated to 8°C and 18°C, respectively. In vitro, isometric ventricular strip preparations demonstrated a similar increase in adrenergic sensitivity with cold-acclimation. The EC50 values for maximal tension development were 2.7x10-7 mol l-1 adrenaline (8°C-acclimated) and 1.1x10-6 mol l-1 adrenaline (18°C-acclimated) when tested at acclimation temperature. This shift in adrenergic sensitivity was a function of the temperature acclimation because changes in bath temperature per se, either from 8°C to 18°C for 8°C- acclimated hearts or from 18°C to 8°C for 18°C-acclimated hearts, had no significant effect on the concentration-response curve for adrenaline. We conducted radioligand binding studies with [125I]iodocyanopindolol and propranolol to quantify the beta-adrenoceptor density (Bmax) of both homogenates and isolated sarcolemmal fractions of ventricles from rainbow trout acclimated to either 8°C or 18°C. The Bmax for isolated sarcolemmal fractions was significantly higher in the 8°C-acclimated group, but the Bmax of ventricular homogenates was not significantly different in the two acclimation groups. No significant differences in dissociation constant (Kd) were apparent in either the homogenates or sarcolemmal fractions. These results suggest that cardiac tissue from rainbow trout acclimated to 8°C has a greater cell surface adrenoceptor population available for beta-antagonist binding. This might explain the heightened cardiac sensitivity to adrenaline observed in situ and in vitro in 8°C-acclimated fish.
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