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Journal of Experimental Biology, Vol 179, Issue 1 63-75, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

AN ANALYSIS OF CONTROL OF THE VENTRICLE OF THE MOLLUSC MERCENARIA MERCENARIA

C. L. Devlin

This study focuses on the ionic mechanisms involved in serotonergic control of the ventricle from the mollusc Mercenaria mercenaria. The effects of calcium (Ca2+), sodium (Na+), potassium (K+), magnesium (Mg2+) and chloride (Cl-) ions on the action of 5-hydroxytryptamine (5-HT) were tested using a sucrose-gap technique. 5-HT increased the amplitude and frequency of the cardiac action potentials (APs) and coupled systolic force in a range of concentrations from the threshold, at 10-10 mol l-1, to 10-6 mol l-1. Low, physiological doses of 5-HT increased the rate of rise and amplitude of the fast rising phase of the AP, and hastened the process of repolarization. Doses of 5-HT higher than 10-5 mol l-1 caused systolic arrest. The action of 5-HT was highly dependent on the presence of physiological levels of extracellular Ca2+. It had a maximal effect on systolic activity in a calcium chloride concentration range of 9-18 mmol l-1. The activity of 5-HT was blocked by treatment with Ca2+-free saline, with inorganic Ca2+ blockers (lanthanum or cobalt) or with an organic Ca2+ entry blocker (verapamil). The effects of 5- HT were potentiated by treatment with barium ions (Ba2+), by a dihydropyridine-sensitive Ca2+ agonist, Bay K 8644, or by a vertebrate Ca2+ entry blocker, diltiazem. Removal of extracellular Na+ or treatment with a Na+ ionophore, monensin, did not significantly affect excitation by 10-6 mol l-1 5-HT; nor did the removal of Cl- or Mg2+. Unlike Ca2+, these three ions probably did not have a critical role during the excitatory action of 5-HT. The excitatory action of 5-HT was not significantly altered by treatment with K+-free saline. When the membrane was depolarized by high-K+ salines, however, 5-HT was unable to elicit any APs or systolic contractions, suggesting that its mechanism may involve voltage-sensitive channels.




© The Company of Biologists Ltd 1993