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Journal of Experimental Biology, Vol 176, Issue 1 233-246, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

TOTAL AMMONIA CONCENTRATION AND pH OF HAEMOLYMPH, PLEON FLUID AND MAXILLARY URINE IN PORCELLIO SCABER LATTREILLE (ISOPODA, ONISCIDEA): RELATIONSHIPS TO AMBIENT HUMIDITY AND WATER VAPOUR UPTAKE

J. C. Wright and M. J. O'Donnell

Ammonia levels and pH were studied in body fluids of Porcellio scaber with special reference to ambient humidity and the timing of pleon fluid secretion and pleopodal ventilation. In high humidity (approximately 96.7 %), concentrations of total ammonia (NH3+NH4+) in haemolymph display pronounced episodic changes, ranging from less than 1 mmol l-1 to more than 50 mmol l-1. Elevated concentrations (>5 mmol l-1) occur in discrete bouts lasting from 40 min to more than 2 h. Concomitant changes in total ammonia levels are seen in pleon fluid and maxillary urine. Overall, mean ammonia concentrations in pleon fluid, but not maxillary urine, are significantly higher than in the haemolymph, which may indicate active secretion of NH4+ in the pleon. Elevated ammonia concentrations are not observed in reduced ambient humidities (85 % and 30 %). Evidence supports a physiological coupling between pleon NH3 volatilization and active water vapour absorption. Pleon fluid and haemolymph pH values are similar, discounting a role of alkalization in NH3 volatilization. Published rates of ammonia excretion are compatible with intermittent volatilization from pleon fluid and with measured NH3 levels during elevations in ammonia concentration.

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